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dc.contributor.authorVajjhala, Parimala R.
dc.contributor.authorCatchpoole, Elizabeth
dc.contributor.authorNguyen, Chau H.
dc.contributor.authorKistler, Carol
dc.contributor.authorMunn, Alan L.
dc.contributor.editorRichard Perham
dc.date.accessioned2017-05-03T15:29:15Z
dc.date.available2017-05-03T15:29:15Z
dc.date.issued2007
dc.date.modified2008-12-08
dc.identifier.issn1742464X
dc.identifier.doi10.1111/j.1742-4658.2007.05736.x
dc.identifier.urihttp://hdl.handle.net/10072/20751
dc.description.abstractDuring endocytic transport, specific integral membrane proteins are sorted into intraluminal vesicles that bud from the limiting membrane of the endosome. This process, known as multivesicular body (MVB) sorting, is important for several important biological processes. Moreover, components of the MVB sorting machinery are implicated in virus budding. During MVB sorting, a cargo protein recruits components of the MVB sorting machinery from cytoplasmic pools and these sequentially assemble on the endosome. Disassembly of these proteins and recycling into the cytoplasm is critical for MVB sorting. Vacuolar protein sorting 4 (Vps4) is an AAA (ATPase associated with a variety of cellular activities) ATPase which has been proposed to play a critical role in disassembly of the MVB sorting machinery. However, the mechanism by which it disassembles the complex is not clear. Vps4 contains an N-terminal microtubule interacting and trafficking (MIT) domain, which has previously been shown to be required for recruitment to endosomes, and a single AAA ATPase domain, the activity of which is required for Vps4 function. In this study we have systematically characterized the interaction of Vps4 with other components of the MVB sorting machinery. We demonstrate that Vps4 interacts directly with Vps2 and Bro1. We also show that a subset of Vps4 interactions is regulated by ATP hydrolysis, and one interaction is regulated by ATP binding. Finally, we show that most proteins interact with the Vps4 MIT domain. Our studies indicate that the MIT domain has a dual role in substrate binding and recruitment to endosomes and indicate that Vps4 disassembles the MVB sorting machinery by direct effects on multiple proteins.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.publisher.placeUK
dc.publisher.urihttps://febs.onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2007.05736.x
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1894
dc.relation.ispartofpageto1907
dc.relation.ispartofissue8
dc.relation.ispartofjournalThe FEBS Journal
dc.relation.ispartofvolume274
dc.rights.retentionY
dc.subject.fieldofresearchMedicinal and biomolecular chemistry
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchMedical biochemistry and metabolomics
dc.subject.fieldofresearchcode3404
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3205
dc.titleVps4 regulates a subset of protein interactions at the multivesicular endosome
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2007
gro.hasfulltextNo Full Text
gro.griffith.authorMunn, Alan L.


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