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  • Pilot studies on the parallel production of soluble mouse proteins in a bacterial expression system

    Author(s)
    Cowieson, NP
    Listwan, P
    Kurz, M
    Aagaard, A
    Ravasi, T
    Wells, C
    Huber, T
    Hume, DA
    Kobe, B
    Martin, JL
    Griffith University Author(s)
    Martin, Jennifer
    Year published
    2005
    Metadata
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    Abstract
    We investigated the parallel production in medium throughput of mouse proteins, using protocols that involved recombinatorial cloning, protein expression screening and batch purification. The methods were scaled up to allow the simultaneous processing of tens or hundreds of protein samples. Scale-up was achieved in two stages. In an initial study, 30 targets were processed manually but with common protocols for all targets. In the second study, these protocols were applied to 96 target proteins that were processed in an automated manner. The success rates at each stage of the study were similar for both the manual and automated ...
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    We investigated the parallel production in medium throughput of mouse proteins, using protocols that involved recombinatorial cloning, protein expression screening and batch purification. The methods were scaled up to allow the simultaneous processing of tens or hundreds of protein samples. Scale-up was achieved in two stages. In an initial study, 30 targets were processed manually but with common protocols for all targets. In the second study, these protocols were applied to 96 target proteins that were processed in an automated manner. The success rates at each stage of the study were similar for both the manual and automated approaches. Overall, 15 of the selected 126 target mouse genes (12%) yielded soluble protein products in a bacterial expression system. This success rate compares favourably with other protein screening projects, particularly for eukaryotic proteins, and could be further improved by modifications at the cloning step
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    Journal Title
    Journal of structural and functional genomics
    Volume
    6
    Issue
    1
    DOI
    https://doi.org/10.1007/s10969-005-0462-7
    Subject
    Environmental sciences
    Biological sciences
    Information and computing sciences
    Publication URI
    http://hdl.handle.net/10072/20923
    Collection
    • Journal articles

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