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dc.contributor.authorA. Wells, Christineen_US
dc.contributor.authorRavasi, Timothyen_US
dc.contributor.authorSultana, Razvanen_US
dc.contributor.authorYagi, Kenen_US
dc.contributor.authorCarninci, Pieroen_US
dc.contributor.authorBono, Hidemasaen_US
dc.contributor.authorFaulkner, Geoffreyen_US
dc.contributor.authorOkazaki, Yasushien_US
dc.contributor.authorQuackenbush, Johnen_US
dc.contributor.authorA. Hume, Daviden_US
dc.contributor.authorGroup, RIKEN GERen_US
dc.contributor.authorMembers, GSLen_US
dc.contributor.authorA. Lyons, Paulen_US
dc.description.abstractThe current RIKEN transcript set represents a significant proportion of the mouse transcriptome but transcripts expressed in the innate and acquired immune systems are poorly represented. In the present study we have assessed the complexity of the transcriptome expressed in mouse macrophages before and after treatment with lipopolysaccharide, a global regulator of macrophage gene expression, using existing RIKEN 19K arrays. By comparison to array profiles of other cells and tissues, we identify a large set of macrophage-enriched genes, many of which have obvious functions in endocytosis and phagocytosis. In addition, a significant number of LPS-inducible genes were identified. The data suggest that macrophages are a complex source of mRNA for transcriptome studies. To assess complexity and identify additional macrophage expressed genes, cDNA libraries were created from purified populations of macrophage and dendritic cells, a functionally related cell type. Sequence analysis revealed a high incidence of novel mRNAs within these cDNA libraries. These studies provide insights into the depths of transcriptional complexity still untapped amongst products of inducible genes, and identify macrophage and dendritic cell populations as a starting point for sampling the inducible mammalian transcriptome.en_US
dc.publisherCold Spring Harbor Laboratory Pressen_US
dc.relation.ispartofjournalGenome Researchen_US
dc.titleContinued Discovery of Transcriptional Units Expressed in Cells of the Mouse Mononuclear Phagocyte Lineageen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.hasfulltextNo Full Text

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