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dc.contributor.authorA. Wells, Christine
dc.contributor.authorRavasi, Timothy
dc.contributor.authorSultana, Razvan
dc.contributor.authorYagi, Ken
dc.contributor.authorCarninci, Piero
dc.contributor.authorBono, Hidemasa
dc.contributor.authorFaulkner, Geoffrey
dc.contributor.authorOkazaki, Yasushi
dc.contributor.authorQuackenbush, John
dc.contributor.authorA. Hume, David
dc.contributor.authorGroup, RIKEN GER
dc.contributor.authorMembers, GSL
dc.contributor.authorA. Lyons, Paul
dc.date.accessioned2017-09-06T02:53:34Z
dc.date.available2017-09-06T02:53:34Z
dc.date.issued2003
dc.identifier.issn10889051en_US
dc.identifier.doi10.1101/gr.1056103en_US
dc.identifier.urihttp://hdl.handle.net/10072/20926
dc.description.abstractThe current RIKEN transcript set represents a significant proportion of the mouse transcriptome but transcripts expressed in the innate and acquired immune systems are poorly represented. In the present study we have assessed the complexity of the transcriptome expressed in mouse macrophages before and after treatment with lipopolysaccharide, a global regulator of macrophage gene expression, using existing RIKEN 19K arrays. By comparison to array profiles of other cells and tissues, we identify a large set of macrophage-enriched genes, many of which have obvious functions in endocytosis and phagocytosis. In addition, a significant number of LPS-inducible genes were identified. The data suggest that macrophages are a complex source of mRNA for transcriptome studies. To assess complexity and identify additional macrophage expressed genes, cDNA libraries were created from purified populations of macrophage and dendritic cells, a functionally related cell type. Sequence analysis revealed a high incidence of novel mRNAs within these cDNA libraries. These studies provide insights into the depths of transcriptional complexity still untapped amongst products of inducible genes, and identify macrophage and dendritic cell populations as a starting point for sampling the inducible mammalian transcriptome.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherCold Spring Harbor Laboratory Pressen_US
dc.publisher.placeUSAen_US
dc.relation.ispartofpagefrom1360en_US
dc.relation.ispartofpageto1365en_US
dc.relation.ispartofjournalGenome Researchen_US
dc.relation.ispartofvolume13en_US
dc.subject.fieldofresearchcode270206en_US
dc.titleContinued Discovery of Transcriptional Units Expressed in Cells of the Mouse Mononuclear Phagocyte Lineageen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
dc.description.versionPublisheden_US
gro.rights.copyrightCopyright 2003 Cold Spring Harbor Laboratory Press. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.en_US
gro.date.issued2015-06-03T02:44:39Z
gro.hasfulltextFull Text


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