Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology.
Author(s)
Mitani, Yasumasa
Lezhava, Alexander
Kawai, Yuki
Kikuchi, Takeshi
Oguchi-Katayama, Atsuko
Kogo, Yasushi
Itoh, Masayoshi
Miyagi, Toru
Takakura, Hideki
Hoshi, Kanako
Kato, Chiaki
Arakawa, Takahiro
Shibata, Kazuhiro
Fukui, Kenji
Masui, Ryoji
Kuramitsu, Seiki
Kiyotani, Kazuma
Chalk, Alistair
Tsunekawa, Katsuhiko
Murakami, Masami
Kamataki, Tetsuya
Oka, Takanori
Shimada, Hiroshi
Cizdziel, Paul
Hayashizaki, Yoshihide
Griffith University Author(s)
Year published
2007
Metadata
Show full item recordAbstract
We developed a rapid single nucleotide polymorphism (SNP) detection system named smart amplification process version 2 (SMAP 2). Because DNA amplification only occurred with a perfect primer match, amplification alone was sufficient to identify the target allele. To achieve the requisite fidelity to support this claim, we used two new and complementary approaches to suppress exponential background DNA amplification that resulted from mispriming events. SMAP 2 is isothermal and achieved SNP detection from whole human blood in 30 min when performed with a new DNA polymerase that was cloned and isolated from Alicyclobacillus ...
View more >We developed a rapid single nucleotide polymorphism (SNP) detection system named smart amplification process version 2 (SMAP 2). Because DNA amplification only occurred with a perfect primer match, amplification alone was sufficient to identify the target allele. To achieve the requisite fidelity to support this claim, we used two new and complementary approaches to suppress exponential background DNA amplification that resulted from mispriming events. SMAP 2 is isothermal and achieved SNP detection from whole human blood in 30 min when performed with a new DNA polymerase that was cloned and isolated from Alicyclobacillus acidocaldarius (Aac pol). Furthermore, to assist the scientific community in configuring SMAP 2 assays, we developed software specific for SMAP 2 primer design. With these new tools, a high-precision and rapid DNA amplification technology becomes available to aid in pharmacogenomic research and molecular-diagnostics applications.
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View more >We developed a rapid single nucleotide polymorphism (SNP) detection system named smart amplification process version 2 (SMAP 2). Because DNA amplification only occurred with a perfect primer match, amplification alone was sufficient to identify the target allele. To achieve the requisite fidelity to support this claim, we used two new and complementary approaches to suppress exponential background DNA amplification that resulted from mispriming events. SMAP 2 is isothermal and achieved SNP detection from whole human blood in 30 min when performed with a new DNA polymerase that was cloned and isolated from Alicyclobacillus acidocaldarius (Aac pol). Furthermore, to assist the scientific community in configuring SMAP 2 assays, we developed software specific for SMAP 2 primer design. With these new tools, a high-precision and rapid DNA amplification technology becomes available to aid in pharmacogenomic research and molecular-diagnostics applications.
View less >
Journal Title
Nature Methods
Volume
4
Issue
3
Publisher URI
Subject
Biological Sciences
Technology
Medical and Health Sciences