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  • Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology.

    Author(s)
    Mitani, Yasumasa
    Lezhava, Alexander
    Kawai, Yuki
    Kikuchi, Takeshi
    Oguchi-Katayama, Atsuko
    Kogo, Yasushi
    Itoh, Masayoshi
    Miyagi, Toru
    Takakura, Hideki
    Hoshi, Kanako
    Kato, Chiaki
    Arakawa, Takahiro
    Shibata, Kazuhiro
    Fukui, Kenji
    Masui, Ryoji
    Kuramitsu, Seiki
    Kiyotani, Kazuma
    Chalk, Alistair
    Tsunekawa, Katsuhiko
    Murakami, Masami
    Kamataki, Tetsuya
    Oka, Takanori
    Shimada, Hiroshi
    Cizdziel, Paul
    Hayashizaki, Yoshihide
    Griffith University Author(s)
    Chalk, Alistair M.
    Year published
    2007
    Metadata
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    Abstract
    We developed a rapid single nucleotide polymorphism (SNP) detection system named smart amplification process version 2 (SMAP 2). Because DNA amplification only occurred with a perfect primer match, amplification alone was sufficient to identify the target allele. To achieve the requisite fidelity to support this claim, we used two new and complementary approaches to suppress exponential background DNA amplification that resulted from mispriming events. SMAP 2 is isothermal and achieved SNP detection from whole human blood in 30 min when performed with a new DNA polymerase that was cloned and isolated from Alicyclobacillus ...
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    We developed a rapid single nucleotide polymorphism (SNP) detection system named smart amplification process version 2 (SMAP 2). Because DNA amplification only occurred with a perfect primer match, amplification alone was sufficient to identify the target allele. To achieve the requisite fidelity to support this claim, we used two new and complementary approaches to suppress exponential background DNA amplification that resulted from mispriming events. SMAP 2 is isothermal and achieved SNP detection from whole human blood in 30 min when performed with a new DNA polymerase that was cloned and isolated from Alicyclobacillus acidocaldarius (Aac pol). Furthermore, to assist the scientific community in configuring SMAP 2 assays, we developed software specific for SMAP 2 primer design. With these new tools, a high-precision and rapid DNA amplification technology becomes available to aid in pharmacogenomic research and molecular-diagnostics applications.
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    Journal Title
    Nature Methods
    Volume
    4
    Issue
    3
    Publisher URI
    http://www.nature.com/nmeth/index.html
    DOI
    https://doi.org/10.1038/nmeth1007
    Subject
    Biological Sciences
    Technology
    Medical and Health Sciences
    Publication URI
    http://hdl.handle.net/10072/20951
    Collection
    • Journal articles

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