dc.contributor.author | Shen, YQ | |
dc.contributor.author | Tiralongo, J | |
dc.contributor.author | Kohla, G | |
dc.contributor.author | Schauer, R | |
dc.date.accessioned | 2017-05-03T14:16:53Z | |
dc.date.available | 2017-05-03T14:16:53Z | |
dc.date.issued | 2004 | |
dc.date.modified | 2009-02-27T06:44:41Z | |
dc.identifier.issn | 1431-6730 | |
dc.identifier.doi | 10.1515/BC.2004.033 | |
dc.identifier.uri | http://hdl.handle.net/10072/21492 | |
dc.description.abstract | The expression of O-acetylated sialic acids in human colonic mucins is developmentally regulated, and a reduction of O-acetylation has been found to be associated with the early stages of colorectal cancer. Despite this, however, little is known about the enzymatic process of sialic acid O-acetylation in human colonic mucosa. Recently, we have reported on a human colon sialate 7(9)-O-acetyltransferase capable of incorporating acetyl groups into sialic acids at the nucleotidesugar level [Shen et al., Biol. Chem. 383 (2002), 307317]. In this report, we show that the CMP-N-acetyl-neuraminic acid (CMPNeu5Ac) and acetyl-CoA (AcCoA) transporters are critical components for the O-acetylation of CMPNeu5Ac in Golgi lumen, with specific inhibition of either transporter leading to a reduction in the formation of CMP-5-Nacetyl-9-O-acetylneuraminic acid (CMP-Neu5,9Ac2). Moreover, the finding that 5-Nacetyl-9-O-acetylneuraminic acid (Neu5,9Ac2) could be transferred from neo-synthesised CMP-Neu5,9Ac2 to endogenous glycoproteins in the same Golgi vesicles, together with the observation that asialofetuin and asialo-human colon mucin are much better acceptors for Neu5,9Ac2 than asialo-bovine submandibular gland mucin, suggests that a sialyltransferase exists that preferentially utilises CMPNeu5,9Ac2 as the donor substrate, transferring Neu5,9Ac2 to terminal Gal߱,3(4)R- residues. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Walter de Gruyter | |
dc.publisher.place | Germany | |
dc.publisher.uri | http://www.reference-global.com/loi/bchm | |
dc.relation.ispartofpagefrom | 145 | |
dc.relation.ispartofpageto | 152 | |
dc.relation.ispartofissue | 2 | |
dc.relation.ispartofjournal | Biological Chemistry | |
dc.relation.ispartofvolume | 385 | |
dc.subject.fieldofresearch | Biochemistry and cell biology | |
dc.subject.fieldofresearchcode | 3101 | |
dc.title | Regulation of sialic acid O-acetylation in human colon mucosa | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.date.issued | 2004 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Tiralongo, Joe | |