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dc.contributor.authorShen, Yanqinen_US
dc.contributor.authorTiralongo, Joeen_US
dc.contributor.authorKohla, Guidoen_US
dc.contributor.authorSchauer, Rolanden_US
dc.date.accessioned2017-05-03T14:16:53Z
dc.date.available2017-05-03T14:16:53Z
dc.date.issued2004en_US
dc.date.modified2009-02-27T06:44:41Z
dc.identifier.issn1431-6730en_US
dc.identifier.doi10.1515/BC.2004.033en_AU
dc.identifier.urihttp://hdl.handle.net/10072/21492
dc.description.abstractThe expression of O-acetylated sialic acids in human colonic mucins is developmentally regulated, and a reduction of O-acetylation has been found to be associated with the early stages of colorectal cancer. Despite this, however, little is known about the enzymatic process of sialic acid O-acetylation in human colonic mucosa. Recently, we have reported on a human colon sialate 7(9)-O-acetyltransferase capable of incorporating acetyl groups into sialic acids at the nucleotidesugar level [Shen et al., Biol. Chem. 383 (2002), 307317]. In this report, we show that the CMP-N-acetyl-neuraminic acid (CMPNeu5Ac) and acetyl-CoA (AcCoA) transporters are critical components for the O-acetylation of CMPNeu5Ac in Golgi lumen, with specific inhibition of either transporter leading to a reduction in the formation of CMP-5-Nacetyl-9-O-acetylneuraminic acid (CMP-Neu5,9Ac2). Moreover, the finding that 5-Nacetyl-9-O-acetylneuraminic acid (Neu5,9Ac2) could be transferred from neo-synthesised CMP-Neu5,9Ac2 to endogenous glycoproteins in the same Golgi vesicles, together with the observation that asialofetuin and asialo-human colon mucin are much better acceptors for Neu5,9Ac2 than asialo-bovine submandibular gland mucin, suggests that a sialyltransferase exists that preferentially utilises CMPNeu5,9Ac2 as the donor substrate, transferring Neu5,9Ac2 to terminal Gal߱,3(4)R- residues.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherWalter de Gruyteren_US
dc.publisher.placeGermanyen_US
dc.publisher.urihttp://www.reference-global.com/loi/bchmen_AU
dc.relation.ispartofpagefrom145en_US
dc.relation.ispartofpageto152en_US
dc.relation.ispartofissue2en_US
dc.relation.ispartofjournalBiological Chemistryen_US
dc.relation.ispartofvolume385en_US
dc.subject.fieldofresearchcode270108en_US
dc.subject.fieldofresearchcode320302en_US
dc.titleRegulation of sialic acid O-acetylation in human colon mucosaen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2004
gro.hasfulltextNo Full Text


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