Show simple item record

dc.contributor.authorL. Dunkley, Margareten_US
dc.contributor.authorRajyaguru, Sonalen_US
dc.contributor.authorMcCue, Amandaen_US
dc.contributor.authorW. Cripps, Allanen_US
dc.contributor.authorM. Kyd, Jennelleen_US
dc.date.accessioned2017-05-03T14:29:37Z
dc.date.available2017-05-03T14:29:37Z
dc.date.issued2003en_US
dc.date.modified2009-03-02T06:51:10Z
dc.identifier.issn09288244en_US
dc.identifier.doi10.1016/S0928-8244(03)00176-7en_AU
dc.identifier.urihttp://hdl.handle.net/10072/21565
dc.description.abstractPseudomonas aeruginosa is an opportunistic Gram-negative pathogen, which causes serious debilitating infections in patients with compromised lung function. The mechanism by which P. aeruginosa is cleared from the lung is not fully defined, although our previous studies have established a role for cellular immunity in protection against P. aeruginosa infections. This study aimed to evaluate the role of P. aeruginosa-specific IgG in protection against P. aeruginosa in a rat model of acute pulmonary infection. Immunoaffinity chromatography was used to purify total rat IgG from rat immune serum (rats immunised with P. aeruginosa) and non-immune serum. Untreated recipient rats were injected intravenously with different concentrations of pure IgG prepared from serum of unimmunised rats (non-immune IgG) or from rats immunised intestinally with killed P. aeruginosa (immune IgG) and infected intratracheally with P. aeruginosa 18 h later. The protective capability of the purified IgG against P. aeruginosa was assessed by measurement of reduction in P. aeruginosa infection in the lung 4 h after instillation of bacteria. Enhanced bacterial clearance induced by IgG was determined to be dose-dependent with a 1 mg dose failing to enhance clearance, whereas 5 mg of immune IgG enhanced clearance from the airways and the lung tissue. Measurement of the IgG1, IgG2a and IgG2b isotypes in serum and the lung lavage following transfer of P. aeruginosa-specific IgG found that all three were present. These results demonstrate that anti-P. aeruginosa IgG can enhance bacterial clearance from the airways in an acute infection and identify an important role for IgG in acute respiratory infections caused by P. aeruginosa.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherElsevier BVen_US
dc.publisher.placeNetherlandsen_US
dc.publisher.urihttp://www.sciencedirect.com/science/journal/09288244en_AU
dc.relation.ispartofpagefrom37en_US
dc.relation.ispartofpageto44en_US
dc.relation.ispartofissue1en_US
dc.relation.ispartofjournalFEMS Immunology and Medical Microbiologyen_US
dc.relation.ispartofvolume39en_US
dc.subject.fieldofresearchcode329999en_US
dc.titlePseudomonas aeruginosa-specific IgG1 and IgG2 subclasses in enhancement of pulmonary clearance following passive immunisation in the raten_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2003
gro.hasfulltextNo Full Text


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record