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dc.contributor.authorTyrer, Peteren_US
dc.contributor.authorRuth Foxwell, A.en_US
dc.contributor.authorKyd, Jennelleen_US
dc.contributor.authorHarvey, Matthewen_US
dc.contributor.authorSizer, Phillipen_US
dc.contributor.authorCripps, Allanen_US
dc.date.accessioned2017-05-03T14:29:22Z
dc.date.available2017-05-03T14:29:22Z
dc.date.issued2003en_US
dc.date.modified2009-03-02T06:51:19Z
dc.identifier.issn0006291Xen_US
dc.identifier.doi10.1016/S0006-291X(02)02631-1en_AU
dc.identifier.urihttp://hdl.handle.net/10072/21567
dc.description.abstractThis study has evaluated an in vitro model of the follicle-associated epithelia that overlie Peyer's patches of the small intestine. The model shares many phenotypic characteristics of M cells in vivo. Co-cultures of the human adenocarcinoma cell line Caco-2 and freshly isolated Peyer's patch cells were established. Fluorescence microscopy and quantitative image analysis were used to validate the model against known markers of M-cell phenotype. Apical expression of alkaline phosphatase was down-regulated in co-cultures and villin was re-distributed from the apical membrane to the cytoplasm. a5߱ integrin was found on the apical surfaces of the monolayers and B and T lymphocytes integrated into the monolayers. Particle transport was temperature-dependent in co-cultures, indicating that a transcytotic route was responsible. This model provides opportunities to study factors that influence M-cell development, assess putative Peyer's patch targeting in oral vaccine technologies, and study intestinal uptake in vitro.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherAcademic Pressen_US
dc.publisher.placeUnited Statesen_US
dc.publisher.urihttp://www.sciencedirect.com/science/journal/0006291Xen_AU
dc.relation.ispartofpagefrom377en_US
dc.relation.ispartofpageto383en_US
dc.relation.ispartofjournalBiochemical and biophysical research communicationsen_US
dc.relation.ispartofvolume299en_US
dc.subject.fieldofresearchcode329999en_US
dc.titleValidation and Quantitation of an in vitro M-cell modelen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2003
gro.hasfulltextNo Full Text


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