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dc.contributor.authorFleming, S.
dc.contributor.authorYu, Fu
dc.contributor.authorMcDonald, M.
dc.contributor.authorMeyers, A.
dc.contributor.authorMontgomery, W.
dc.contributor.authorSmith, F.
dc.contributor.authorNicholson, D.
dc.date.accessioned2017-05-03T15:00:51Z
dc.date.available2017-05-03T15:00:51Z
dc.date.issued2004
dc.date.modified2009-03-04T06:54:13Z
dc.identifier.issn1040452X
dc.identifier.doi10.1002/mrd.20049
dc.identifier.urihttp://hdl.handle.net/10072/21603
dc.description.abstractSperm surface protein PH-20 expression was studied during spermatogenesis in pubertal and adult sheep, using molecular and histological methods. The effects of 24 hr of insulation raising scrotal temperatures to 39 degrees C on PH-20 expression in ejaculated sheep sperm were also determined. A 282 nt cDNA fragment of ovine PH-20 was identified in total RNA extracts of sheep testes, which exhibited 76% identity at the nucleotide level with the equivalent region of the human sequence. Ovine PH-20 mRNA and immunoreactivity were identified only in adult ram testis and not in peri-pubertal ram testis tubules lacking round spermatids, nor in adult sheep brain, pituitary, heart, spleen, lung, liver, kidney, epididymis, or ovary. Ovine PH-20 protein was distributed predominantly on the postacrosomal membrane and was also present on the anterior membrane of the sperm head in fresh, unheated sheep semen. Scrotal heating caused a significant, transient decrease in the percentage of PH-20 immunoreactive sperm, but did not change the pattern of PH-20 staining on the sperm head. The results strongly suggest that ovine PH-20 is postmeiotically expressed in haploid germ cells in sheep testis and is arrayed on the membrane of the mature ovine spermatozoon. Scrotal heating appears to have few effects on PH-20 expression and distribution on ejaculated sperm.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherJohn Wiley & Sons, Inc.
dc.publisher.placeHoboken, NJ
dc.relation.ispartofpagefrom103
dc.relation.ispartofpageto114
dc.relation.ispartofissue1
dc.relation.ispartofjournalMolecular Reproduction and Development
dc.relation.ispartofvolume68
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchPaediatrics and Reproductive Medicine
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1114
dc.titleEffects of scrotal heating on sperm surface protein PH-20 expression in sheep
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2004
gro.hasfulltextNo Full Text
gro.griffith.authorFleming, Jean S.


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