dc.contributor.author | Fleming, S. | |
dc.contributor.author | Yu, Fu | |
dc.contributor.author | McDonald, M. | |
dc.contributor.author | Meyers, A. | |
dc.contributor.author | Montgomery, W. | |
dc.contributor.author | Smith, F. | |
dc.contributor.author | Nicholson, D. | |
dc.date.accessioned | 2017-05-03T15:00:51Z | |
dc.date.available | 2017-05-03T15:00:51Z | |
dc.date.issued | 2004 | |
dc.date.modified | 2009-03-04T06:54:13Z | |
dc.identifier.issn | 1040452X | |
dc.identifier.doi | 10.1002/mrd.20049 | |
dc.identifier.uri | http://hdl.handle.net/10072/21603 | |
dc.description.abstract | Sperm surface protein PH-20 expression was studied during spermatogenesis in pubertal and adult sheep, using molecular and histological methods. The effects of 24 hr of insulation raising scrotal temperatures to 39 degrees C on PH-20 expression in ejaculated sheep sperm were also determined. A 282 nt cDNA fragment of ovine PH-20 was identified in total RNA extracts of sheep testes, which exhibited 76% identity at the nucleotide level with the equivalent region of the human sequence. Ovine PH-20 mRNA and immunoreactivity were identified only in adult ram testis and not in peri-pubertal ram testis tubules lacking round spermatids, nor in adult sheep brain, pituitary, heart, spleen, lung, liver, kidney, epididymis, or ovary. Ovine PH-20 protein was distributed predominantly on the postacrosomal membrane and was also present on the anterior membrane of the sperm head in fresh, unheated sheep semen. Scrotal heating caused a significant, transient decrease in the percentage of PH-20 immunoreactive sperm, but did not change the pattern of PH-20 staining on the sperm head. The results strongly suggest that ovine PH-20 is postmeiotically expressed in haploid germ cells in sheep testis and is arrayed on the membrane of the mature ovine spermatozoon. Scrotal heating appears to have few effects on PH-20 expression and distribution on ejaculated sperm. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | John Wiley & Sons, Inc. | |
dc.publisher.place | Hoboken, NJ | |
dc.relation.ispartofpagefrom | 103 | |
dc.relation.ispartofpageto | 114 | |
dc.relation.ispartofissue | 1 | |
dc.relation.ispartofjournal | Molecular Reproduction and Development | |
dc.relation.ispartofvolume | 68 | |
dc.subject.fieldofresearch | Biochemistry and Cell Biology | |
dc.subject.fieldofresearch | Paediatrics and Reproductive Medicine | |
dc.subject.fieldofresearchcode | 0601 | |
dc.subject.fieldofresearchcode | 1114 | |
dc.title | Effects of scrotal heating on sperm surface protein PH-20 expression in sheep | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.date.issued | 2004 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Fleming, Jean S. | |