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  • Balancing shiftwork and life outside work: Do 12-h shifts make a difference?

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    50939_1.pdf (138.7Kb)
    Author(s)
    Loudoun, Rebecca
    Griffith University Author(s)
    Loudoun, Rebecca J.
    Year published
    2008
    Metadata
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    Abstract
    Amounts of ߁-activin, ߃-activin, activin receptor subunits ActRIIA and ActRIIB mRNA, and ߁- and ߃-activin subunit protein immunoreactivity were investigated in male Lewis rats, either untreated or after 5 or 10 weeks of CCl4 treatment to induce cirrhosis. Apoptosis was assessed histologically and with an in situ cell death detection kit (TUNEL). Reverse transcription and polymerase chain reaction were used to evaluate mRNA levels. Activin ߁- and ߃-subunit immunoreactivity was studied by immunohistochemistry using specific monoclonal antibodies. Hepatocellular apoptosis (P<0.001), increased ߁- and ߃-activin mRNAs (three- to ...
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    Amounts of ߁-activin, ߃-activin, activin receptor subunits ActRIIA and ActRIIB mRNA, and ߁- and ߃-activin subunit protein immunoreactivity were investigated in male Lewis rats, either untreated or after 5 or 10 weeks of CCl4 treatment to induce cirrhosis. Apoptosis was assessed histologically and with an in situ cell death detection kit (TUNEL). Reverse transcription and polymerase chain reaction were used to evaluate mRNA levels. Activin ߁- and ߃-subunit immunoreactivity was studied by immunohistochemistry using specific monoclonal antibodies. Hepatocellular apoptosis (P<0.001), increased ߁- and ߃-activin mRNAs (three- to fourfold; P<0.01) and increased ߁- and ߃-activin tissue immunoreactivity were evident, whereas ActRIIA mRNA concentrations fell (30%; P<0.01) after 5 weeks of CCl4 treatment. The mRNA concentrations at 10 weeks were not significantly different from controls, despite extensive hepatic nodule formation. We conclude that the increased activin subunit expression is associated with apoptosis, rather than hepatic fibrosis and nodule formation.
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    Journal Title
    Applied Ergonomics
    Volume
    39
    Issue
    5
    Publisher URI
    http://www.elsevier.com/locate/mce
    DOI
    https://doi.org/10.1016/j.apergo.2007.12.004
    Copyright Statement
    © 2008 Elsevier. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
    Subject
    Sports science and exercise
    Medical physiology
    Design
    Publication URI
    http://hdl.handle.net/10072/21664
    Collection
    • Journal articles

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