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dc.contributor.authorForrest, Alistair R. R.
dc.contributor.authorRavasi, Timothy
dc.contributor.authorTaylor, Darrin
dc.contributor.authorHuber, Thomas
dc.contributor.authorHume, David
dc.contributor.authorRIKEN GER Group
dc.contributor.authorGSL Members
dc.contributor.authorGrimmond, Sean
dc.date.accessioned2017-09-06T00:41:11Z
dc.date.available2017-09-06T00:41:11Z
dc.date.issued2003
dc.identifier.issn10889051
dc.identifier.doi10.1101/gr.954803
dc.identifier.urihttp://hdl.handle.net/10072/21722
dc.description.abstractWith the completion of the human and mouse genome sequences, the task now turns to identifying their encoded transcripts and assigning gene function. In this study, we have undertaken a computational approach to identify and classify all of the protein kinases and phosphatases present in the mouse gene complement. A nonredundant set of these sequences was produced by mining Ensembl gene predictions and publicly available cDNA sequences with a panel of InterPro domains. This approach identified 561 candidate protein kinases and 162 candidate protein phosphatases. This cohort was then analyzed using TribeMCL protein sequence similarity clustering followed by CLUSTALV alignment and hierarchical tree generation. This approach allowed us to (1) distinguish between true members of the protein kinase and phosphatase families and enzymes of related biochemistry, (2) determine the structure of the families, and (3) suggest functions for previously uncharacterized members. The classifications obtained by this approach were in good agreement with previous schemes and allowed us to demonstrate domain associations with a number of clusters. Finally, we comment on the complementary nature of cDNA and genome-based gene detection and the impact of the FANTOM2 transcriptome project.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoen_US
dc.publisherCold Spring Harbor Laboratory Press, Publications Department
dc.publisher.placeUnited States
dc.relation.ispartofpagefrom1443
dc.relation.ispartofpageto1454
dc.relation.ispartofissue6B
dc.relation.ispartofjournalGenome Research
dc.relation.ispartofvolume13
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode11
dc.titlePhosphoregulators: Protein Kinases and Protein Phosphatases of Mouse
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionPublished
gro.rights.copyright© 2003 Cold Spring Harbor Laboratory Press. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.date.issued2015-06-03T02:45:23Z
gro.hasfulltextFull Text
gro.griffith.authorForrest, Alistair RR.


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