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dc.contributor.authorNowlan, Biancaen_US
dc.contributor.authorS. Dodia, Mitalen_US
dc.contributor.authorP. Singh, Satyaen_US
dc.contributor.authorPatel, Bharaten_US
dc.contributor.editorDr. Peter Kaempfer (Editor-in-Chief)en_US
dc.date.accessioned2017-04-24T14:51:19Z
dc.date.available2017-04-24T14:51:19Z
dc.date.issued2006en_US
dc.date.modified2009-04-03T08:52:31Z
dc.identifier.issn14665026en_US
dc.identifier.doi10.1099/ijs.0.63861-0en_AU
dc.identifier.urihttp://hdl.handle.net/10072/22063
dc.description.abstractA strictly aerobic, rod-shaped bacterium (0.6-0.8x2-3 孩, designated strain Kh10-101T, was isolated from a saltpan (22࠱5' N, 69࠱' E) in the vicinity of Port Okha, India. The creamish pigmented colonies of strain Kh10-101T were round, flat and translucent with irregular margins and a smooth surface. The strain possessed up to three subpolar flagella, and was motile by a corkscrew motion. The strain grew optimally at 37 à(temperature growth range 25-40 é in a complex glucose-containing medium with 5 % NaCl (NaCl growth range 0-10 %) at pH 9 (pH growth range pH 7-10), indicating that it was a mesophilic halotolerant alkaliphile. The strain was sensitive to lincomycin, meticillin, cefuroxime and cephalexin, but resistant to gentamicin, tetracycline and cotrimazine. Spores were not detected and cells were heat sensitive. The isolate metabolized a range of carbohydrates and hydrolysed casein, gelatin and starch. Growth was not observed on aromatic compounds, Tween 40 or Tween 80. Nitrate was not reduced and catalase was produced. Electron microscopic examination of thin sections revealed a single thick Gram-positive cell wall. The DNA G+C content was 41ᱠmol%. Phylogenetic analyses of the 16S rRNA gene sequence revealed that strain Kh10-101T was a member of the sixth rRNA group of the genus Bacillus, which includes alkalitolerant, alkaliphilic and halotolerant species. The halotolerant obligate alkaliphile Bacillus krulwichiae is the closest relative of strain Kh10-101T (96 % similarity) but a number of phenotypic differences suggest that strain Kh10-101T (=JCM 13040T=ATCC BAA-1137T) should be designated the type strain of a new species, for which the name Bacillus okhensis sp. nov. is proposed.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherSociety for General Microbiologyen_US
dc.publisher.placeUnited Kingdomen_US
dc.publisher.urihttp://ijs.sgmjournals.org/en_AU
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom1073en_US
dc.relation.ispartofpageto1077en_US
dc.relation.ispartofissue5en_US
dc.relation.ispartofjournalInternational Journal of Systematic and Evolutionary Microbiologyen_US
dc.relation.ispartofvolume56en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry not elsewhere classifieden_US
dc.subject.fieldofresearchcode030499en_US
dc.titleBacillus okhensis sp. nov., a halotolerant and alkalitolerant bacterium from an Indian saltpanen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2006
gro.hasfulltextNo Full Text


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