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dc.contributor.authorSergeev, A.
dc.contributor.authorPetrishchenko, V.
dc.contributor.authorPyankov, O.
dc.contributor.authorPyankova, O.
dc.contributor.authorShishkina, L.
dc.contributor.authorSergeev, A.
dc.contributor.authorKachko, A.
dc.contributor.authorKiselyev, N.
dc.contributor.authorTernovoi, V.
dc.contributor.authorSvyatchenko, V.
dc.contributor.editorDmitrii K Lvov
dc.date.accessioned2017-05-03T16:55:52Z
dc.date.available2017-05-03T16:55:52Z
dc.date.issued2003
dc.date.modified2009-04-16T06:53:28Z
dc.identifier.issn05074088
dc.identifier.urihttp://hdl.handle.net/10072/22174
dc.description.abstractThe results of polymerase chain reaction and of DNA sequencing of the Adel2 mutant variant of adenovirus serotype 5, passaged 10 times and capable of selectively infecting and lysing the p53-deficient human tumor cells, are indicative of a high stability of its genotype and of the phenotypic properties acquired by it in successive passage on 293 cells. The absence of admixtures of wild-type adenovirus was clearly shown in the cultivation and passage processes. It was revealed in an experimental analysis of virus-productive properties of the studied continuous cell culture 293 by using the method of multilayer cultivation, that the maximal Adel2 yield is obtained at the 50% cytopathic effect. Virus doses, that are effective for cell-culture contamination, are within a range of 100-10 TCPE50 per cell. In order to spare the viral material, the infecting dose of 10 TCPE50 per cell was chosen to infect a cell monolayer.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageRussian
dc.language.isorus
dc.publisherIzdatel'stvo Meditsina
dc.publisher.placeRussian Federation
dc.relation.ispartofpagefrom30
dc.relation.ispartofpageto33
dc.relation.ispartofissue6
dc.relation.ispartofjournalVoprosy Virusologii
dc.relation.ispartofvolume48
dc.subject.fieldofresearchcode270304
dc.titleA possibility to cultivate the mutant Adel2 adenovirus variant on the cell-293 culture
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2003
gro.hasfulltextNo Full Text
gro.griffith.authorPyankov, Oleg


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