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dc.contributor.authorSato, T
dc.contributor.authorKanai, Y
dc.contributor.authorNoma, T
dc.contributor.authorKanai-Azuma, M
dc.contributor.authorTaya, S
dc.contributor.authorMatsui, T
dc.contributor.authorIshii, M
dc.contributor.authorKawakami, H
dc.contributor.authorKurohmaru, M
dc.contributor.authorKaibuchi, K
dc.contributor.authorWood, SA
dc.contributor.authorHayashi, Y
dc.date.accessioned2017-05-03T16:55:31Z
dc.date.available2017-05-03T16:55:31Z
dc.date.issued2004
dc.date.modified2009-04-16T06:53:51Z
dc.identifier.issn1470-1626
dc.identifier.doi10.1530/rep.1.00060
dc.identifier.urihttp://hdl.handle.net/10072/22181
dc.description.abstractUsp9x, an X-linked deubiquitylating enzyme, is stage dependently expressed in the supporting cells (i.e. Sertoli cells and granulosa cells) and germ cells during mouse gametogenesis. Af-6, a cell junction protein, has been identified as a substrate of Usp9x, suggesting a possible association between Usp9x and Af-6 in spermatogenesis and oogenesis. In this study, we examined the expression pattern of Af-6 and Usp9x and their intracellular localization in testes and ovaries of mice treated with or without pregnant mare serum gonadotropin (PMSG), an FSH-like hormone. In both testes and ovaries, Af-6 expression was predominantly observed in supporting cells, as well as in steroidogenic cells, but not in any germ cells. In Sertoli cells, Af-6 was continuously expressed throughout postnatal and adult stages, where both Af-6 and Usp9x were enriched at the sites of Sertoli-Sertoli and Sertoli-spermatid junctions especially at stages XI-VI. In the granulosa cells, Af-6, as well as Usp9x, was highly expressed in primordial and primary follicles, but its expression rapidly decreased after the late-secondary follicle stage. Interestingly, in PMSG-treated mice, the expression levels of Af-6 and Usp9x were synchronously enhanced, slightly in Sertoli cells and strongly in granulosa cells of the late-secondary and Graafian follicles. Such closely correlated expression patterns between Af-6 and Usp9x clearly suggest that Af-6 may be deubiquitylated by Usp9x in both Sertoli and granulosa cells. It further suggests that the post-translational regulation of Af-6 by Usp9x may be one potential pathway to control the cell adhesion dynamics in mammalian gametogenesis.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBioScientifica Ltd.
dc.publisher.placeUnited Kingdom
dc.relation.ispartofpagefrom583
dc.relation.ispartofpageto594
dc.relation.ispartofissue5
dc.relation.ispartofjournalReproduction
dc.relation.ispartofvolume128
dc.subject.fieldofresearchBiochemistry and cell biology not elsewhere classified
dc.subject.fieldofresearchZoology
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchcode310199
dc.subject.fieldofresearchcode3109
dc.subject.fieldofresearchcode3202
dc.titleA close correlation in the expression patterns of Af-6 and Usp9x in Sertoli and granulosa cells of mouse testis and ovary
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2004
gro.hasfulltextNo Full Text
gro.griffith.authorWood, Stephen A.


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