dc.contributor.author | Sato, T | |
dc.contributor.author | Kanai, Y | |
dc.contributor.author | Noma, T | |
dc.contributor.author | Kanai-Azuma, M | |
dc.contributor.author | Taya, S | |
dc.contributor.author | Matsui, T | |
dc.contributor.author | Ishii, M | |
dc.contributor.author | Kawakami, H | |
dc.contributor.author | Kurohmaru, M | |
dc.contributor.author | Kaibuchi, K | |
dc.contributor.author | Wood, SA | |
dc.contributor.author | Hayashi, Y | |
dc.date.accessioned | 2017-05-03T16:55:31Z | |
dc.date.available | 2017-05-03T16:55:31Z | |
dc.date.issued | 2004 | |
dc.date.modified | 2009-04-16T06:53:51Z | |
dc.identifier.issn | 1470-1626 | |
dc.identifier.doi | 10.1530/rep.1.00060 | |
dc.identifier.uri | http://hdl.handle.net/10072/22181 | |
dc.description.abstract | Usp9x, an X-linked deubiquitylating enzyme, is stage dependently expressed in the supporting cells (i.e. Sertoli cells and granulosa cells) and germ cells during mouse gametogenesis. Af-6, a cell junction protein, has been identified as a substrate of Usp9x, suggesting a possible association between Usp9x and Af-6 in spermatogenesis and oogenesis. In this study, we examined the expression pattern of Af-6 and Usp9x and their intracellular localization in testes and ovaries of mice treated with or without pregnant mare serum gonadotropin (PMSG), an FSH-like hormone. In both testes and ovaries, Af-6 expression was predominantly observed in supporting cells, as well as in steroidogenic cells, but not in any germ cells. In Sertoli cells, Af-6 was continuously expressed throughout postnatal and adult stages, where both Af-6 and Usp9x were enriched at the sites of Sertoli-Sertoli and Sertoli-spermatid junctions especially at stages XI-VI. In the granulosa cells, Af-6, as well as Usp9x, was highly expressed in primordial and primary follicles, but its expression rapidly decreased after the late-secondary follicle stage. Interestingly, in PMSG-treated mice, the expression levels of Af-6 and Usp9x were synchronously enhanced, slightly in Sertoli cells and strongly in granulosa cells of the late-secondary and Graafian follicles. Such closely correlated expression patterns between Af-6 and Usp9x clearly suggest that Af-6 may be deubiquitylated by Usp9x in both Sertoli and granulosa cells. It further suggests that the post-translational regulation of Af-6 by Usp9x may be one potential pathway to control the cell adhesion dynamics in mammalian gametogenesis. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | BioScientifica Ltd. | |
dc.publisher.place | United Kingdom | |
dc.relation.ispartofpagefrom | 583 | |
dc.relation.ispartofpageto | 594 | |
dc.relation.ispartofissue | 5 | |
dc.relation.ispartofjournal | Reproduction | |
dc.relation.ispartofvolume | 128 | |
dc.subject.fieldofresearch | Biochemistry and cell biology not elsewhere classified | |
dc.subject.fieldofresearch | Zoology | |
dc.subject.fieldofresearch | Clinical sciences | |
dc.subject.fieldofresearchcode | 310199 | |
dc.subject.fieldofresearchcode | 3109 | |
dc.subject.fieldofresearchcode | 3202 | |
dc.title | A close correlation in the expression patterns of Af-6 and Usp9x in Sertoli and granulosa cells of mouse testis and ovary | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.date.issued | 2004 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Wood, Stephen A. | |