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  • A Comprehensive Transcript Map of the Mouse Gnas Imprinted Complex

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    Author(s)
    Holmes, Rebecca
    Williamson, Christine
    Peters, Jo
    Denny, Paul
    Group, RIKEN GER
    Members, GSL
    Wells, Christine
    Griffith University Author(s)
    Wells, Christine
    Year published
    2003
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    Abstract
    The recent publication of the FANTOM mouse transcriptome has provided a unique opportunity to study the diversity of transcripts arising from a single gene locus. We have focused on the Gnas complex, as imprinting loci themselves provide unique insights into transcriptional regulation. Thirteen full-length cDNAs from the FANTOM2 set were mapped to the Gnas locus. These represented one previously described transcript and 12 putative new transcripts. Of these, eight were found to be differentially expressed from either the maternal or paternal allele. Two clones extended Nespas in the 3_ direction, providing evidence of antisense ...
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    The recent publication of the FANTOM mouse transcriptome has provided a unique opportunity to study the diversity of transcripts arising from a single gene locus. We have focused on the Gnas complex, as imprinting loci themselves provide unique insights into transcriptional regulation. Thirteen full-length cDNAs from the FANTOM2 set were mapped to the Gnas locus. These represented one previously described transcript and 12 putative new transcripts. Of these, eight were found to be differentially expressed from either the maternal or paternal allele. Two clones extended Nespas in the 3_ direction, providing evidence of antisense transcription spanning a 30-kb genomic region from a single allele. The transcripts were summarized into six transcriptional units, Nespas, Nesp, Gnasxl, F7, exon 1A, and Gnas. The resolution of the Gnas transcript map by the FANTOM2 clones revealed a pattern of alternate splicing. In addition to the transcripts described previously as splicing onto exon 2 of Gnas, each new sense transcript had an alternate short 3_UTR independent of Gnas. Both spliced and unspliced variants of the new imprinted sense transcripts were found. Whereas the functional significance of these alternate transcripts is not known, the availability of the FANTOM clones has provided remarkable insights into the repertoire of transcripts in the Gnas complex locus.
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    Journal Title
    Genome Research
    Volume
    13
    Issue
    6B
    DOI
    https://doi.org/10.1101/gr.955503
    Copyright Statement
    © The Author(s) 2003. This is an Open Access article distributed under the terms of the Creative Commons CC-BY-NC License (Attribution-NonCommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
    Subject
    Genetics not elsewhere classified
    Biological Sciences
    Medical and Health Sciences
    Publication URI
    http://hdl.handle.net/10072/22241
    Collection
    • Journal articles

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