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  • Matrix metalloproteinase localisation by in situ-RT-PCR in archival human breast biopsy material

    Author(s)
    Haupt, LM
    Irving, RE
    Weinstein, SR
    Irving, MG
    Griffiths, LR
    Griffith University Author(s)
    Haupt, Larisa
    Griffiths, Lyn
    Weinstein, Stephen R.
    Irving, Rachel
    Year published
    2008
    Metadata
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    Abstract
    Utilising archival human breast cancer biopsy material we examined the stromal/epithelial interactions of several matrix metalloproteinases (MMPs) using in situ-RT-PCR (IS-RT-PCR). In breast cancer, the stromal/epithelial interactions that occur, and the site of production of these proteases, are central to understanding their role in invasive and metastatic processes. We examined MT1-MMP (MMP-14, membrane type-1-MMP), MMP-1 (interstitial collagenase) and MMP-3 (stromelysin-1) for their localisation profile in progressive breast cancer biopsy material (poorly differentiated invasive breast carcinoma (PDIBC), invasive breast ...
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    Utilising archival human breast cancer biopsy material we examined the stromal/epithelial interactions of several matrix metalloproteinases (MMPs) using in situ-RT-PCR (IS-RT-PCR). In breast cancer, the stromal/epithelial interactions that occur, and the site of production of these proteases, are central to understanding their role in invasive and metastatic processes. We examined MT1-MMP (MMP-14, membrane type-1-MMP), MMP-1 (interstitial collagenase) and MMP-3 (stromelysin-1) for their localisation profile in progressive breast cancer biopsy material (poorly differentiated invasive breast carcinoma (PDIBC), invasive breast carcinomas (IBC) and lymph node metastases (LNM)). Expression of MT1-MMP, MMP-1 and MMP-3 was observed in both the tumour epithelial and surrounding stromal cells in most tissue sections examined. MT1-MMP expression was predominantly localised to the tumour component in the pre-invasive lesions. MMP-1 gene expression was relatively well distributed between both tissue compartments, while MMP-3 demonstrated highest expression levels in the stromal tissue surrounding the epithelial tumour cells. The results demonstrate the ability to distinguish compartmental gene expression profiles using IS-RT-PCR. Further, we suggest a role for MT1-MMP in early tumour progression, expression of MMP-1 during metastasis and focal expression pattern of MMP-3 in areas of expansion. These expression profiles may provide markers for early breast cancer diagnoses and present potential therapeutic targets.
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    Journal Title
    Molecular and Cellular Probes
    Volume
    22
    Issue
    2
    Publisher URI
    https://www.sciencedirect.com/science/article/pii/S0890850807000552
    DOI
    https://doi.org/10.1016/j.mcp.2007.06.009
    Subject
    Medicinal and biomolecular chemistry
    Biochemistry and cell biology
    Cellular interactions (incl. adhesion, matrix, cell wall)
    Publication URI
    http://hdl.handle.net/10072/22405
    Collection
    • Journal articles

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