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dc.contributor.authorHaupt, LM
dc.contributor.authorIrving, RE
dc.contributor.authorWeinstein, SR
dc.contributor.authorIrving, MG
dc.contributor.authorGriffiths, LR
dc.date.accessioned2017-05-03T15:27:43Z
dc.date.available2017-05-03T15:27:43Z
dc.date.issued2008
dc.date.modified2009-11-13T06:37:36Z
dc.identifier.issn0890-8508
dc.identifier.doi10.1016/j.mcp.2007.06.009
dc.identifier.urihttp://hdl.handle.net/10072/22405
dc.description.abstractUtilising archival human breast cancer biopsy material we examined the stromal/epithelial interactions of several matrix metalloproteinases (MMPs) using in situ-RT-PCR (IS-RT-PCR). In breast cancer, the stromal/epithelial interactions that occur, and the site of production of these proteases, are central to understanding their role in invasive and metastatic processes. We examined MT1-MMP (MMP-14, membrane type-1-MMP), MMP-1 (interstitial collagenase) and MMP-3 (stromelysin-1) for their localisation profile in progressive breast cancer biopsy material (poorly differentiated invasive breast carcinoma (PDIBC), invasive breast carcinomas (IBC) and lymph node metastases (LNM)). Expression of MT1-MMP, MMP-1 and MMP-3 was observed in both the tumour epithelial and surrounding stromal cells in most tissue sections examined. MT1-MMP expression was predominantly localised to the tumour component in the pre-invasive lesions. MMP-1 gene expression was relatively well distributed between both tissue compartments, while MMP-3 demonstrated highest expression levels in the stromal tissue surrounding the epithelial tumour cells. The results demonstrate the ability to distinguish compartmental gene expression profiles using IS-RT-PCR. Further, we suggest a role for MT1-MMP in early tumour progression, expression of MMP-1 during metastasis and focal expression pattern of MMP-3 in areas of expansion. These expression profiles may provide markers for early breast cancer diagnoses and present potential therapeutic targets.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeUnited Kingdom
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S0890850807000552
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom83
dc.relation.ispartofpageto89
dc.relation.ispartofissue2
dc.relation.ispartofjournalMolecular and Cellular Probes
dc.relation.ispartofvolume22
dc.rights.retentionY
dc.subject.fieldofresearchCellular Interactions (incl. Adhesion, Matrix, Cell Wall)
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchcode060106
dc.subject.fieldofresearchcode0304
dc.subject.fieldofresearchcode0601
dc.titleMatrix metalloproteinase localisation by in situ-RT-PCR in archival human breast biopsy material
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2008
gro.hasfulltextNo Full Text
gro.griffith.authorHaupt, Larisa
gro.griffith.authorGriffiths, Lyn
gro.griffith.authorWeinstein, Stephen R.
gro.griffith.authorIrving, Rachel


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