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dc.contributor.authorMorita, Eri
dc.contributor.authorWatanabe, Yasuhiro
dc.contributor.authorIshimoto, Miho
dc.contributor.authorNakano, Toshiya
dc.contributor.authorKitayama, Michio
dc.contributor.authorYasui, Kenichi
dc.contributor.authorFukada, Yasuyo
dc.contributor.authorDoi, Koji
dc.contributor.authorKarunaratne, Asanka
dc.contributor.authorMurrell, Wayne G
dc.contributor.authorSutharsan, Ratneswary
dc.contributor.authorMackay-Sim, Alan
dc.contributor.authorHata, Yoshio
dc.contributor.authorNakashima, Kenji
dc.contributor.editorS Gilman (Editor-in-Chief)
dc.date.accessioned2017-05-03T15:24:51Z
dc.date.available2017-05-03T15:24:51Z
dc.date.issued2008
dc.date.modified2009-09-22T05:51:20Z
dc.identifier.issn0014-4886
dc.identifier.doi10.1016/j.expneurol.2008.07.011
dc.identifier.urihttp://hdl.handle.net/10072/22515
dc.description.abstractAmyotrophic lateral sclerosis (ALS) is a lethal neurodegenerative disease, which selectively affects motor neurons throughout the central nervous system. The extensive distribution of motor neurons is an obstacle to applying cell transplantation therapy for the treatment of ALS. To overcome this problem, we developed a cell transplantation method via the fourth cerebral ventricle in mice. We used mouse olfactory ensheathing cells (OECs) and rat mesenchymal stem cells (MSCs) as donor cells. OECs are reported to promote regeneration and remyelination in the spinal cord, while MSCs have a capability to differentiate into several types of specific cells including neural cells. Furthermore both types of cells can be relatively easily obtained by biopsy in human. Initially, we confirmed the safety of the operative procedure and broad distribution of grafted cells in the spinal cord using wild-type mice. After transplantation, OECs distributed widely and survived as long as 100 days after transplantation, with a time-dependent depletion of cell number. In ALS model mice, OEC transplantation revealed no adverse effects but no significant differences in clinical evaluation were found between OEC-treated and non-transplanted animals. After MSC transplantation into the ALS model mice, females, but not males, showed a statistically longer disease duration than the non-transplanted controls. We conclude that intrathecal ansplantation could be a promising way to deliver donor cells to the central nervous system. Further experiments to elucidate relevant conditions for optimal outcomes are required.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAcademic Press / Elsevier
dc.publisher.placeUnited States
dc.publisher.urihttp://www.sciencedirect.com/science/journal/00144886
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom431
dc.relation.ispartofpageto438
dc.relation.ispartofissue2
dc.relation.ispartofjournalExperimental Neurology
dc.relation.ispartofvolume213
dc.rights.retentionY
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchNeurosciences
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode3209
dc.titleA novel cell transplantation protocol and its application to an ALS mouse model
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2008
gro.hasfulltextNo Full Text
gro.griffith.authorMackay-Sim, Alan
gro.griffith.authorMurrell, Wayne G.
gro.griffith.authorKarunaratne, Asanka M.
gro.griffith.authorSutharsan, Ratneswary


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