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  • Desiccation and cryopreservation tolerance of near mature seeds of Citrus garrawayae

    Author(s)
    Hamilton, KN
    Ashmore, SE
    Drew, RA
    Griffith University Author(s)
    Ashmore, Sarah
    Drew, Roderick A.
    Hamilton, Kim N.
    Year published
    2008
    Metadata
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    Abstract
    The effect of desiccation and liquid nitrogen exposure on seeds of Citrus garrawayae F.M. Bailey was investigated to facilitate ex situ conservation. Near mature seeds demonstrated tolerance to desiccation to low moisture contents (8% moisture content) and liquid nitrogen exposure. Thermal analysis of seeds revealed the absence of phase transitions attributable to ice formation / melting in seeds dried to low moisture contents which coincided with maximal germination after liquid nitrogen exposure. Germination of seeds was achieved in vitro, seedlings were successfully acclimatized to the shade house, and these demonstrated ...
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    The effect of desiccation and liquid nitrogen exposure on seeds of Citrus garrawayae F.M. Bailey was investigated to facilitate ex situ conservation. Near mature seeds demonstrated tolerance to desiccation to low moisture contents (8% moisture content) and liquid nitrogen exposure. Thermal analysis of seeds revealed the absence of phase transitions attributable to ice formation / melting in seeds dried to low moisture contents which coincided with maximal germination after liquid nitrogen exposure. Germination of seeds was achieved in vitro, seedlings were successfully acclimatized to the shade house, and these demonstrated phenotypically normal growth in the field. This study therefore reports a protocol for the cryopreservation of C. garrawayae seeds which, in conjunction with a previously published micropropagation system, allows in vitro propagation of the limited seed material available in this rare listed species (e.g. for restoration purposes).
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    Journal Title
    Seed Science and Technology
    Volume
    36
    Issue
    1
    Publisher URI
    http://www.seedtest.org/en/seed_science_and_technology_content---1--1084.html
    Copyright Statement
    © 2008 International Seed Testing Association. Self-archiving of the author-manuscript version is not yet supported by this publisher. Please refer to the journal link for access to the definitive, published version or contact the author for more information.
    Subject
    Plant biology
    Crop and pasture production
    History, heritage and archaeology
    Publication URI
    http://hdl.handle.net/10072/22548
    Collection
    • Journal articles

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