Show simple item record

dc.contributor.authorBingley, Johnen_US
dc.contributor.authorP. Hayward, Ianen_US
dc.contributor.authorA. Girjes, Adeeben_US
dc.contributor.authorR. Campbell, Gordonen_US
dc.contributor.authorE. Humphries, Donalden_US
dc.contributor.authorL. Stow, Jenniferen_US
dc.contributor.authorH. Campbell, Julieen_US
dc.date.accessioned2017-04-24T11:31:35Z
dc.date.available2017-04-24T11:31:35Z
dc.date.issued2002en_US
dc.date.modified2009-04-20T04:46:39Z
dc.identifier.issn00182214en_US
dc.identifier.doi10.1023/A:1020938430120en_AU
dc.identifier.urihttp://hdl.handle.net/10072/22605
dc.description.abstractHeparan sulphate is an important mediator in determining vascular smooth muscle cell (SMC) phenotype. The sulphation pattern of the heparan sulphate chains is critical to their function. We have examined the initial step in the biosynthesis of the sulphated domains mediated by the enzyme heparan sulphate N-deacetylase/N-sulphotransferase (NDST). Rabbit aortic SMC in primary culture exhibited NDST enzyme activity and expressed NDST-1 in their Golgi apparatus, with maximal expression in SMC 2 days after dispersal in primary culture confirmed by Western blot analysis. Endothelial cells, macrophages and fibroblasts expressed NDST-1 but had generally less intense staining than SMC, although SMC expression decreased with culture. The uninjured rat aorta also showed widespread expression of NDST-1. After balloon de-endothelialisation, NDST-1 could not be detected in SMC of the neointima in the early stages of neointimal formation, but was re-expressed at later time points (after 12 weeks). In human coronary arteries, SMC of the media and the diffuse intimal thickening expressed NDST-1, while SMC in the atherosclerotic plaque were negative for NDST-1. We conclude that SMC may regulate their heparan sulphate sulphation at the level of expression of the enzyme heparan sulphate NDST in a manner related to their phenotypic state.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherSpringeren_US
dc.publisher.placeNetherlandsen_US
dc.relation.ispartofpagefrom131en_US
dc.relation.ispartofpageto137en_US
dc.relation.ispartofissue3-4en_US
dc.relation.ispartofjournalThe Histochemical Journalen_US
dc.relation.ispartofvolume34en_US
dc.subject.fieldofresearchcode321003en_US
dc.titleExpression of heparan sulphate N-deacetylase/N-sulphotransferase by vascular smooth muscle cells.en_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2002
gro.hasfulltextNo Full Text


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record