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dc.contributor.authorHolmes, Roger S
dc.contributor.authorChan, Jeannie
dc.contributor.authorCox, Laura A
dc.contributor.authorMurphy, William J
dc.contributor.authorVandeBerg, John L
dc.date.accessioned2017-05-03T16:56:04Z
dc.date.available2017-05-03T16:56:04Z
dc.date.issued2008
dc.date.modified2009-05-13T07:46:04Z
dc.identifier.issn1471-2148
dc.identifier.doi10.1186/1471-2148-8-54
dc.identifier.urihttp://hdl.handle.net/10072/22929
dc.description.abstractBackground Carboxylesterases (CES) perform diverse metabolic roles in mammalian organisms in the detoxification of a broad range of drugs and xenobiotics and may also serve in specific roles in lipid, cholesterol, pheromone and lung surfactant metabolism. Five CES families have been reported in mammals with human CES1 and CES2 the most extensively studied. Here we describe the genetics, expression and phylogeny of CES isozymes in the opossum and report on the sequences and locations of CES1, CES2 and CES6 'like' genes within two gene clusters on chromosome one. We also discuss the likely sequence of gene duplication events generating multiple CES genes during vertebrate evolution. Results We report a cDNA sequence for an opossum CES and present evidence for CES1 and CES2 like genes expressed in opossum liver and intestine and for distinct gene locations of five opossum CES genes,CES1, CES2.1, CES2.2, CES2.3 and CES6, on chromosome 1. Phylogenetic and sequence alignment studies compared the predicted amino acid sequences for opossum CES with those for human, mouse, chicken, frog, salmon and Drosophila CES gene products. Phylogenetic analyses produced congruent phylogenetic trees depicting a rapid early diversification into at least five distinct CES gene family clusters: CES2, CES1, CES7, CES3, and CES6. Molecular divergence estimates based on a Bayesian relaxed clock approach revealed an origin for the five mammalian CES gene families between 328-378 MYA. Conclusion The deduced amino acid sequence for an opossum cDNA was consistent with its identity as a mammalian CES2 gene product (designated CES2.1). Distinct gene locations for opossum CES1 (1: 446,222,550-446,274,850), three CES2 genes (1: 677,773,395-677,927,030) and a CES6 gene (1: 677,585,520-677,730,419) were observed on chromosome 1. Opossum CES1 and multiple CES2 genes were expressed in liver and intestine. Amino acid sequences for opossum CES1 and three CES2 gene products revealed conserved residues previously reported for human CES1 involved in catalysis, ligand binding, tertiary structure and organelle localization. Phylogenetic studies indicated the gene duplication events which generated ancestral mammalian CES genes predated the common ancestor for marsupial and eutherian mammals, and appear to coincide with the early diversification of tetrapods.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent474312 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherBioMed Central Ltd
dc.publisher.placeUK
dc.publisher.urihttp://www.biomedcentral.com/1471-2148/8/54/abstract
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto13
dc.relation.ispartofjournalBMC Evolutionary Biology
dc.relation.ispartofvolume8
dc.rights.retentionY
dc.subject.fieldofresearchGenetics not elsewhere classified
dc.subject.fieldofresearchEvolutionary Biology
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchcode060499
dc.subject.fieldofresearchcode0603
dc.subject.fieldofresearchcode0604
dc.titleOpossum carboxylesterases: sequences, phylogeny and evidence for CES gene duplication events predating the marsupial-eutherian common ancestor
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/2.0
gro.rights.copyright© 2008 Holmes et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.date.issued2008
gro.hasfulltextFull Text
gro.griffith.authorHolmes, Roger S.


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