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dc.contributor.authorKeenan, JI
dc.contributor.authorDavis, KA
dc.contributor.authorBeaugie, CR
dc.contributor.authorMcGovern, JJ
dc.contributor.authorMoran, AP
dc.contributor.editorOtto Holst
dc.date.accessioned2017-05-03T16:56:58Z
dc.date.available2017-05-03T16:56:58Z
dc.date.issued2008
dc.date.modified2012-05-29T22:09:06Z
dc.identifier.issn1753-4259
dc.identifier.doi10.1177/1753425908096857
dc.identifier.urihttp://hdl.handle.net/10072/23240
dc.description.abstractOuter membrane vesicles (OMVs) shed from the gastroduodenal pathogen Helicobacter pylori have measurable effects on epithelial cell responses. The aim of this study was to determine the effect of iron availability, and its basis, on the extent and nature of lipopolysaccharide (LPS) produced on H. pylori OMVs and their parental bacterial cells. Electrophoretic, immunoblotting and structural analyses revealed that LPSs of bacterial cells grown under iron-limited conditions were notably shorter than those of bacteria and OMVs obtained from iron-replete conditions. Structural analysis and serological probing showed that LPSs of iron-replete cells and OMVs expressed O-chains of Lewisx with a terminal Lewisy unit, whereas Lewisy expression was notably reduced on bacteria and OMVs from iron-limiting conditions. Unlike the O-chain, the core oligosaccharide and lipid A moieties of iron-replete and iron-limited bacteria and their OMVs were similar. Quantitatively, shed OMVs from iron-replete bacteria were found to be LPSenriched, whereas shed OMVs from iron-limited bacteria had a significantly reduced content of LPS. These differences were linked to bacterial ATP levels. Since iron availability affects the extent and nature of LPS expressed by H. pylori, host iron status may contribute to H. pylori pathogenesis.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSage Science Press (UK)
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom279
dc.relation.ispartofpageto290
dc.relation.ispartofissue5
dc.relation.ispartofjournalInnate Immunity
dc.relation.ispartofvolume14
dc.rights.retentionY
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchBacteriology
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchCellular immunology
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode310701
dc.subject.fieldofresearchcode3204
dc.subject.fieldofresearchcode320404
dc.titleAlterations in Helicobacter pylori outer membrane and outer membrane vesicle-associated lipopolysaccharides under iron-limiting growth conditions
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2008
gro.hasfulltextNo Full Text
gro.griffith.authorMoran, Anthony P.


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