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  • Image processing and reconstruction of cultured neuron skeletons

    Author(s)
    Yu, Donggang
    D. Pham, Tuan
    Yan, Hong
    S. Jin, Jesse
    Luo, Suhuai
    Crane, Denis
    Griffith University Author(s)
    Crane, Denis I.
    Year published
    2008
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    Abstract
    One approach to investigating neural death is through systematic studies of the changing morphology of cultured brain neurons in response to cellular challenges. Image segmentation and neuron skeleton reconstruction methods developed to date to analyze such changes have been limited by the low contrast of cells. In this paper we present new algorithms that successfully circumvent these problems. The binary method is based on logical analysis of grey and distance difference of images. The spurious regions are detected and removed through use of a hierarchical window filter. The skeletons of binary cell images are extracted. ...
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    One approach to investigating neural death is through systematic studies of the changing morphology of cultured brain neurons in response to cellular challenges. Image segmentation and neuron skeleton reconstruction methods developed to date to analyze such changes have been limited by the low contrast of cells. In this paper we present new algorithms that successfully circumvent these problems. The binary method is based on logical analysis of grey and distance difference of images. The spurious regions are detected and removed through use of a hierarchical window filter. The skeletons of binary cell images are extracted. The extension direction and connection points of broken cell skeletons are automatically determined, and broken neural skeletons are reconstructed. The spurious strokes are deleted based on cell prior knowledge. The reconstructed skeletons are processed furthermore by filling holes, smoothing and extracting new skeletons. The final constructed neuron skeletons are analyzed and calculated to find the length and morphology of skeleton branches automatically. The efficacy of the developed algorithms is demonstrated here through a test of cultured brain neurons from newborn mice.
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    Journal Title
    International Journal of Hybrid Intelligent Systems
    Volume
    5
    Issue
    4
    Publisher URI
    http://content.iospress.com/articles/international-journal-of-hybrid-intelligent-systems/his00081
    Subject
    Image Processing
    Artificial Intelligence and Image Processing
    Cognitive Sciences
    Publication URI
    http://hdl.handle.net/10072/23577
    Collection
    • Journal articles

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