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dc.contributor.authorCreeper, F
dc.contributor.authorLichanska, AM
dc.contributor.authorMarshall, RI
dc.contributor.authorSeymour, GJ
dc.contributor.authorIvanovski, S
dc.date.accessioned2017-05-03T15:16:59Z
dc.date.available2017-05-03T15:16:59Z
dc.date.issued2009
dc.date.modified2009-07-27T07:01:42Z
dc.identifier.issn0022-3484
dc.identifier.doi10.1111/j.1600-0765.2008.01125.x
dc.identifier.urihttp://hdl.handle.net/10072/24882
dc.description.abstractBackground and Objective: Platelet-rich plasma is used to deliver growth factors, in a safe and convenient manner, for enhancing bone and periodontal regeneration. However, conflicting reports regarding its effectiveness suggest that further study of the relevant cellular mechanisms is required. The aim of this study was to investigate the in vitro effect of platelet-rich plasma on osteoblasts and periodontal ligament cell function. Material and Methods: Various concentrations of platelet-rich plasma (100, 50, 20 and 10%) and platelet-poor plasma, obtained from human donors, were applied to primary cultures of human osteoblasts and periodontal ligament cells. [3H]-Thymidine incorporation, crystal violet staining and MTT assays were utilized to assess DNA synthesis and proliferation. Migration was determined by assessing the cell response to a concentration gradient, while differentiation was assessed using Alazarin Red staining. Results: Platelet-rich plasma and platelet-poor plasma had stimulatory effects on the migration of both human osteoblasts and periodontal ligament cells. At 24 h, DNA synthesis was suppressed by the application of the various concentrations of platelet-rich plasma, but over a 5-d period, a beneficial effect on proliferation was observed, especially in response to 50% platelet-rich plasma. Platelet-poor plasma resulted in the greatest enhancement of cellular proliferation for both cell types. At a concentration of 50%, platelet-rich plasma and platelet-poor plasma facilitated differentiation of both cell types. Conclusion: Platelet-rich plasma can exert a positive effect on osteoblast and periodontal ligament cell function, but this effect is concentration specific with maximal concentrations not necessarily resulting in optimal outcomes. Platelet-poor plasma also appears to have the ability to promote wound healing-associated cell function.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent178180 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley-Blackwell Munksgaard
dc.publisher.placeDenmark
dc.publisher.urihttps://onlinelibrary.wiley.com/doi/10.1111/j.1600-0765.2008.01125.x
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom258
dc.relation.ispartofpageto265
dc.relation.ispartofjournalJournal of Periodontal Research
dc.relation.ispartofvolume44
dc.rights.retentionY
dc.subject.fieldofresearchPeriodontics
dc.subject.fieldofresearchDentistry
dc.subject.fieldofresearchcode110508
dc.subject.fieldofresearchcode1105
dc.titleThe effect of platelet-rich plasma on osteoblast and periodontal ligament cell migration, proliferation and differentiation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2009 Blackwell Publishing. This is a pre-peer reviewed, author-produced PDF of an article accepted for publication in the Journal of Periodontal Research. The definitive version is available at www.interscience.wiley.com
gro.date.issued2009
gro.hasfulltextFull Text
gro.griffith.authorIvanovski, Saso


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