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  • Human Melanoblasts in Culture: Expression of BRN2 and Synergistic Regulation by Fibroblast Growth Factor-2, Stem Cell Factor, and Endothelin-3

    Author(s)
    L. Cook, Anthony
    D. Donatien, Philippe
    G. Smith, Aaron
    Murphy, Mark
    K. Jones, Malcolm
    Herlyn, Meenhard
    C. Bennett, Dorothy
    Helen Leonard, J.
    Sturm, Richard A.
    Griffith University Author(s)
    Cook, Anthony
    Year published
    2003
    Metadata
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    Abstract
    The BRN2 transcription factor (POU3F2, N-Oct-3) has been implicated in development of the melanocytic lineage and in melanoma. Using a low calcium medium supplemented with stem cell factor, fibroblast growth factor-2, endothelin-3 and cholera toxin, we have established and partially characterised human melanocyte precursor cells, which are unpigmented, contain immature melanosomes and lack L-dihydroxyphenylalanine reactivity. Melanoblast cultures expressed high levels of BRN2 compared to melanocytes, which decreased to a level similar to that of melanocytes when cultured in medium that contained phorbol ester but lacked ...
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    The BRN2 transcription factor (POU3F2, N-Oct-3) has been implicated in development of the melanocytic lineage and in melanoma. Using a low calcium medium supplemented with stem cell factor, fibroblast growth factor-2, endothelin-3 and cholera toxin, we have established and partially characterised human melanocyte precursor cells, which are unpigmented, contain immature melanosomes and lack L-dihydroxyphenylalanine reactivity. Melanoblast cultures expressed high levels of BRN2 compared to melanocytes, which decreased to a level similar to that of melanocytes when cultured in medium that contained phorbol ester but lacked endothelin-3, stem cell factor and fibroblast growth factor-2. This decrease in BRN2 accompanied a positive L-dihydroxyphenylalanine reaction and induction of melanosome maturation consistent with melanoblast differentiation seen during development. Culture of primary melanocytes in low calcium medium supplemented with stem cell factor, fibroblast growth factor-2 and endothelin-3 caused an increase in BRN2 protein levels with a concomitant change to a melanoblast-like morphology. Synergism between any two of these growth factors was required for BRN2 protein induction, whereas all three factors were required to alter melanocyte morphology and for maximal BRN2 protein expression. These finding implicate BRN2 as an early marker of melanoblasts that may contribute to the hierarchy of melanocytic gene control.
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    Journal Title
    Journal of Investigative Dermatology
    Volume
    101
    Issue
    5
    Publisher URI
    http://www.nature.com/jid/
    DOI
    https://doi.org/10.1046/j.1523-1747.2003.12562.x
    Subject
    Cell Development, Proliferation and Death
    Clinical Sciences
    Oncology and Carcinogenesis
    Publication URI
    http://hdl.handle.net/10072/24884
    Collection
    • Journal articles

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