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dc.contributor.authorL. Cook, Anthony
dc.contributor.authorD. Donatien, Philippe
dc.contributor.authorG. Smith, Aaron
dc.contributor.authorMurphy, Mark
dc.contributor.authorK. Jones, Malcolm
dc.contributor.authorHerlyn, Meenhard
dc.contributor.authorC. Bennett, Dorothy
dc.contributor.authorHelen Leonard, J.
dc.contributor.authorSturm, Richard A.
dc.date.accessioned2017-05-03T16:57:51Z
dc.date.available2017-05-03T16:57:51Z
dc.date.issued2003
dc.date.modified2009-07-27T07:01:48Z
dc.identifier.issn0022202X
dc.identifier.doi10.1046/j.1523-1747.2003.12562.x
dc.identifier.urihttp://hdl.handle.net/10072/24884
dc.description.abstractThe BRN2 transcription factor (POU3F2, N-Oct-3) has been implicated in development of the melanocytic lineage and in melanoma. Using a low calcium medium supplemented with stem cell factor, fibroblast growth factor-2, endothelin-3 and cholera toxin, we have established and partially characterised human melanocyte precursor cells, which are unpigmented, contain immature melanosomes and lack L-dihydroxyphenylalanine reactivity. Melanoblast cultures expressed high levels of BRN2 compared to melanocytes, which decreased to a level similar to that of melanocytes when cultured in medium that contained phorbol ester but lacked endothelin-3, stem cell factor and fibroblast growth factor-2. This decrease in BRN2 accompanied a positive L-dihydroxyphenylalanine reaction and induction of melanosome maturation consistent with melanoblast differentiation seen during development. Culture of primary melanocytes in low calcium medium supplemented with stem cell factor, fibroblast growth factor-2 and endothelin-3 caused an increase in BRN2 protein levels with a concomitant change to a melanoblast-like morphology. Synergism between any two of these growth factors was required for BRN2 protein induction, whereas all three factors were required to alter melanocyte morphology and for maximal BRN2 protein expression. These finding implicate BRN2 as an early marker of melanoblasts that may contribute to the hierarchy of melanocytic gene control.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.publisher.placeUnited States
dc.publisher.urihttp://www.nature.com/jid/
dc.relation.ispartofpagefrom1150
dc.relation.ispartofpageto1159
dc.relation.ispartofissue5
dc.relation.ispartofjournalJournal of Investigative Dermatology
dc.relation.ispartofvolume101
dc.subject.fieldofresearchCell Development, Proliferation and Death
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchOncology and Carcinogenesis
dc.subject.fieldofresearchcode060103
dc.subject.fieldofresearchcode1103
dc.subject.fieldofresearchcode1112
dc.titleHuman Melanoblasts in Culture: Expression of BRN2 and Synergistic Regulation by Fibroblast Growth Factor-2, Stem Cell Factor, and Endothelin-3
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2003
gro.hasfulltextNo Full Text
gro.griffith.authorCook, Anthony


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