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dc.contributor.authorSawant, PD
dc.contributor.authorWatson, G
dc.contributor.authorNicolau, DV
dc.contributor.authorNicolau, DV
dc.contributor.editorDV Nicolau, J Enderlein, RC Leif, DL Farkas
dc.date.accessioned2017-05-03T16:57:22Z
dc.date.available2017-05-03T16:57:22Z
dc.date.issued2004
dc.identifier.issn0277-786X
dc.identifier.doi10.1117/12.543444
dc.identifier.urihttp://hdl.handle.net/10072/25363
dc.description.abstractThe immobilization and hybridization processes of DNA strands on poly-l-lysine (PL) covered surfaces have been studied using the atomic force microscopy (AFM) in a topographic mode. The statistical analysis of topographic surfaces showed an increase in the Z-threshold with additions of single strand DNA (ssDNA) and the complimentary DNA (ccDNA). Also no drastic change of statistical fractal dimension (slope of the log-log perimeter-area plot) is observed when comparing the PL-surfaces coated with ssDNA and ccDNA. These two results suggest that ssDNA strands are successfully immobilized and spatially hybridized with ccDNA on the PL surface and the growth of hybridized ccDNA occurs mainly in the vertical dimension. The methods described here are good candidates for the detection of DNA hybridization, especially in the context of DNA nanoarrays.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSPIE
dc.publisher.placeBellingham, WA, USA
dc.relation.ispartofconferencenameImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II
dc.relation.ispartofconferencetitleProceedings of SPIE - The International Society for Optical Engineering
dc.relation.ispartofdatefrom2004-01-27
dc.relation.ispartofdateto2004-01-28
dc.relation.ispartoflocationSan Jose, CA, USA
dc.relation.ispartofpagefrom122
dc.relation.ispartofpageto130
dc.relation.ispartofvolume5322
dc.subject.fieldofresearchcode291599
dc.titleAn AFM study of the hierarchical DNA immobilization/hybridization processes on surfaces
dc.typeConference output
dc.type.descriptionE1 - Conferences
dc.type.codeE - Conference Publications
gro.date.issued2015-05-31T22:20:54Z
gro.hasfulltextNo Full Text
gro.griffith.authorWatson, Gregory S.


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    Contains papers delivered by Griffith authors at national and international conferences.

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