Show simple item record

dc.contributor.authorJun, Fan Yong
dc.contributor.authorLong, Li
dc.contributor.authorJian, Xu
dc.contributor.authorLian, Yu
dc.date.accessioned2017-05-03T16:58:26Z
dc.date.available2017-05-03T16:58:26Z
dc.date.issued2004
dc.date.modified2009-08-30T23:14:58Z
dc.identifier.issn02539977
dc.identifier.urihttp://hdl.handle.net/10072/25458
dc.description.abstractThe oligodendrocyte-myelin glycoprotein (OMgp), a phosphatidylinositol-linked membrane glycoprotein expressed in the brain, was found to be an inhibitor of neurite outgrowth and axonal regeneration after brain injury. Several observation suggest that the LRR domain of OMgp may play an important role in inhibition of cell proliferation. Here we report an analysis of the domain requirement of OMgp in neurite outgrowth inhibition .The distinct domain of OMgp was subcloned into pGEX-4T vector and GST-fusion protein comprised distinct domain of OMgp were expressed. The protein were used to culture with NgR-expressing CHO and hippocampal neurons. Results show that the fragment consisting of the S/T domain and C-terminal of OMgp can not bind with NgR-expressing CHO cell. The fragment representing of LRR can bind with NgR-expressing CHO and inhibit the neurite outgrowth of primary neurons. It can also pull down NgR from mouse brain. The results suggest that the LRR domain of OMgp is required and sufficient for binding of NgR and neurite outgrowth inhibition.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherShanghai Scientific and Technical Publishers
dc.publisher.placeShanghai, China
dc.publisher.urihttp://www.cjcb.org/portal/ezl_show.jsp?ID=808
dc.relation.ispartofpagefrom290
dc.relation.ispartofpageto296
dc.relation.ispartofissue3
dc.relation.ispartofjournalChinese Journal of Cell Biology
dc.relation.ispartofvolume26
dc.subject.fieldofresearchBiomedical Engineering not elsewhere classified
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry not elsewhere classified
dc.subject.fieldofresearchcode090399
dc.subject.fieldofresearchcode030499
dc.titleDistinct Effects of OMgp Domains in Neurite Outgrowth Inhibition
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2004
gro.hasfulltextNo Full Text
gro.griffith.authorFan, Yongjun


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record