OMgp LRR181-228: The Predominant Domain of OMgp in Neurite Outgrowth Inhibition
Author(s)
Fan, Yong-Jun
Xu, Jian
Li, Long
Zhang, Chao-Zheng
Wan, Wang-Jun
Yu, Lian
Griffith University Author(s)
Year published
2004
Metadata
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Several observations suggest that the leucine-rich repeats (LRR) domain of oligodendrocyte-myelin glycoprotein (OMgp) may play an important role in inhibition of neurite outgrowth and axonal regeneration after brain injury. To better understand structure-function relationships for the OMgp LRR domain and its effects in neurite regeneration, distinct OMgp leucine-rich repeat were deleted by PCR based site-directed mutagenesis. The gene deleted OMgp LRR fragments were expressed with GST protein and the expressed proteins were used to culture with NgR-expressing CHO and hippocampal neurons. Results show that the deletion of ...
View more >Several observations suggest that the leucine-rich repeats (LRR) domain of oligodendrocyte-myelin glycoprotein (OMgp) may play an important role in inhibition of neurite outgrowth and axonal regeneration after brain injury. To better understand structure-function relationships for the OMgp LRR domain and its effects in neurite regeneration, distinct OMgp leucine-rich repeat were deleted by PCR based site-directed mutagenesis. The gene deleted OMgp LRR fragments were expressed with GST protein and the expressed proteins were used to culture with NgR-expressing CHO and hippocampal neurons. Results show that the deletion of OMgp amino acid residues 25-56, 57-133,134-180 did not interrupt its effects in binding NgR-expressing CHO and inhibiting hippocampal neurons growth. But the fragment deleted amino acid residues 181-228 can only bind with NgR-expressing CHO and lost the effect of neurite outgrowth inhibition. Results suggest that the OMgp amino acid 181-228 is the predominant domain in neurite outgrowth inhibition of OMgp. The OMgp LRR fragment without amino acid residues 181-228 may be a potent reagent for encouraging regeneration in the adult central nervous system following injury.
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View more >Several observations suggest that the leucine-rich repeats (LRR) domain of oligodendrocyte-myelin glycoprotein (OMgp) may play an important role in inhibition of neurite outgrowth and axonal regeneration after brain injury. To better understand structure-function relationships for the OMgp LRR domain and its effects in neurite regeneration, distinct OMgp leucine-rich repeat were deleted by PCR based site-directed mutagenesis. The gene deleted OMgp LRR fragments were expressed with GST protein and the expressed proteins were used to culture with NgR-expressing CHO and hippocampal neurons. Results show that the deletion of OMgp amino acid residues 25-56, 57-133,134-180 did not interrupt its effects in binding NgR-expressing CHO and inhibiting hippocampal neurons growth. But the fragment deleted amino acid residues 181-228 can only bind with NgR-expressing CHO and lost the effect of neurite outgrowth inhibition. Results suggest that the OMgp amino acid 181-228 is the predominant domain in neurite outgrowth inhibition of OMgp. The OMgp LRR fragment without amino acid residues 181-228 may be a potent reagent for encouraging regeneration in the adult central nervous system following injury.
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Journal Title
Chinese Journal of Cell Biology
Volume
26
Issue
5
Publisher URI
Subject
Biomedical Engineering not elsewhere classified
Medicinal and Biomolecular Chemistry not elsewhere classified