• myGriffith
    • Staff portal
    • Contact Us⌄
      • Future student enquiries 1800 677 728
      • Current student enquiries 1800 154 055
      • International enquiries +61 7 3735 6425
      • General enquiries 07 3735 7111
      • Online enquiries
      • Staff phonebook
    View Item 
    •   Home
    • Griffith Research Online
    • Journal articles
    • View Item
    • Home
    • Griffith Research Online
    • Journal articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

  • All of Griffith Research Online
    • Communities & Collections
    • Authors
    • By Issue Date
    • Titles
  • This Collection
    • Authors
    • By Issue Date
    • Titles
  • Statistics

  • Most Popular Items
  • Statistics by Country
  • Most Popular Authors
  • Support

  • Contact us
  • FAQs
  • Admin login

  • Login
  • Nasal-associated lymphoid tissue and olfactory epithelium as portals of entry for Burkholderia pseudomallei in murine melioidosis

    Thumbnail
    View/Open
    56186_1.pdf (809.7Kb)
    Author(s)
    Owen, Suzzanne
    Batzloff, Michael
    Chehrehasa, Fatemeh
    Meedeniya, Adrian
    Casart Quintero, Yveth
    Logue, Carie-Anne
    Hirst, Robert
    Peak, Ian
    Mackay-Sim, Alan
    Beacham, Ifor
    Griffith University Author(s)
    Beacham, Ifor R.
    Mackay-Sim, Alan
    Logue, Carie-Anne
    Peak, Ian
    Owen, Suzzanne J.
    Hirst, Robert G.
    Casart Quintero, Yveth
    Meedeniya, Adrian C.
    Chehrehasa, Fatemeh
    Year published
    2009
    Metadata
    Show full item record
    Abstract
    BACKGROUND: Burkholderia pseudomallei, the causative agent of melioidosis, is generally considered to be acquired via inhalation of dust or water droplets from the environment. In this study, we show that infection of the nasal mucosa is potentially an important portal of entry in melioidosis. METHODS: After intranasal inoculation of mice, infection was monitored by bioluminescence imaging and by immunohistological analysis of coronal sections. The bacterial loads in organ and tissue specimens were also monitored. RESULTS: Bioluminescence imaging showed colonization and replication in the nasal cavity, including the ...
    View more >
    BACKGROUND: Burkholderia pseudomallei, the causative agent of melioidosis, is generally considered to be acquired via inhalation of dust or water droplets from the environment. In this study, we show that infection of the nasal mucosa is potentially an important portal of entry in melioidosis. METHODS: After intranasal inoculation of mice, infection was monitored by bioluminescence imaging and by immunohistological analysis of coronal sections. The bacterial loads in organ and tissue specimens were also monitored. RESULTS: Bioluminescence imaging showed colonization and replication in the nasal cavity, including the nasal-associated lymphoid tissue (NALT). Analysis of coronal sections and immunofluorescence microscopy further demonstrated the presence of infection in the respiratory epithelium and the olfactory epithelium (including associated nerve bundles), as well as in the NALT. Of significance, the olfactory epithelium and the brain were rapidly infected before bacteria were detected in blood, and a capsule-deficient mutant infected the brain without significantly infecting blood. CONCLUSIONS: These data suggest that the olfactory nerve is the route of entry into the brain and that this route of entry may be paralleled in cases of human neurologic melioidosis. This study focuses attention on the upper respiratory tract as a portal of entry, specifically focusing on NALT as a route for the development of systemic infection via the bloodstream and on the olfactory epithelium as a direct route to the brain.
    View less >
    Journal Title
    Journal of Infectious Diseases
    Volume
    199
    Issue
    12
    Publisher URI
    https://doi.org/10.1086/599210
    DOI
    https://doi.org/10.1086/599210
    Copyright Statement
    © 2009 by University of Chicago Press. The attached file is reproduced here in accordance with the copyright policy of the publisher. First published in The Journal of Geology. Please refer to the journal's website for access to the definitive, published version.
    Subject
    Infectious Agents
    Central Nervous System
    Biological Sciences
    Medical and Health Sciences
    Publication URI
    http://hdl.handle.net/10072/25756
    Collection
    • Journal articles

    Footer

    Disclaimer

    • Privacy policy
    • Copyright matters
    • CRICOS Provider - 00233E
    • TEQSA: PRV12076

    Tagline

    • Gold Coast
    • Logan
    • Brisbane - Queensland, Australia
    First Peoples of Australia
    • Aboriginal
    • Torres Strait Islander