Show simple item record

dc.contributor.authorN. Peart, Jasonen_US
dc.contributor.authorJ. Gross, Garretten_US
dc.date.accessioned2017-04-24T10:09:12Z
dc.date.available2017-04-24T10:09:12Z
dc.date.issued2005en_US
dc.date.modified2009-09-28T06:51:17Z
dc.identifier.issn03008428en_US
dc.identifier.doi10.1007/s00395-005-0526-7en_AU
dc.identifier.urihttp://hdl.handle.net/10072/25829
dc.description.abstractAdenosine kinase phosphorylates adenosine to AMP, the primary pathway for adenosine metabolism under basal conditions. Inhibition of adenosine kinase results in a site-specific increase in interstitial adenosine. Using a rat model of myocardial infarction, we examined the protective effects of adenosine kinase inhibition. Male Sprague-Dawley rats underwent 30 min regional occlusion followed by 90 min reperfusion. Infarct size, expressed as a percent of the area-at-risk, IS/AAR(%), was 58.0 ᠲ.1 % in untreated rats. Pretreatment with the adenosine kinase inhibitor, 5-iodotubercidin (1 mg/kg), limited infarct development to 37.5ᳮ7% (P < 0.001). The A1 adenosine receptor (A1AR) antagonist, DPCPX (100 姯kg), abolished the infarct-sparing effect of 5-iodotubercidin (IS, 62.8 ᠱ.3%). Similarly, the A3 adenosine receptor (A3AR) antagonist, MRS-1523 (2 mg/kg), and the d-opioid receptor (DOR) antagonist, BNTX, (1 mg/kg) abolished the reduction of IS produced by iodotubercidin. Pretreatment with the ROS scavenger, 2-MPG (20 mg/kg), or the PKC-d antagonist, rottlerin (0.3 mg/kg) also abolished iodotubercidin-mediated cardioprotection. Furthermore, pretreatment with 5-HD, a mitochondrial KATP (mitoKATP) channel inhibitor, but not the sarcolemmal KATP channel blocker, HMR-1098, abrogated the beneficial effects of adenosine kinase inhibition (IS, 59.5 ᠳ.8%). These data suggest that inhibition of adenosine kinase is effective in reducing infarct development via A1AR, A3AR and DOR activation. Data also suggest that this protection is mediated via ROS, PKC-d and mitoKATP channels.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherDr. Dietrich Steinkopff Verlagen_US
dc.publisher.placeGermanyen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom328en_US
dc.relation.ispartofpageto336en_US
dc.relation.ispartofjournalBasic Research in Cardiologyen_US
dc.relation.ispartofvolume100en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchcode320503en_US
dc.titleCardioprotection following adenosine kinase inhibition in rat heartsen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2005
gro.hasfulltextNo Full Text


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record