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dc.contributor.authorGross, Eric
dc.contributor.authorPeart, Jason
dc.contributor.authorHsu, Anna
dc.contributor.authorAuchampach, John
dc.contributor.authorGross, Garrett
dc.date.accessioned2017-05-03T13:03:44Z
dc.date.available2017-05-03T13:03:44Z
dc.date.issued2005
dc.identifier.issn03636135
dc.identifier.doi10.1152/ajpheart.00918.2004
dc.identifier.urihttp://hdl.handle.net/10072/25842
dc.description.abstractSelective d-opioid agonists produce delayed cardioprotection that lasts for 24-48 h in rats; however, the maximum length of the cardioprotective window is unclear. In this study, we attempted to prolong the cardioprotective window using a unique d-opioid agonist, fentanyl isothiocyanate (FIT), which binds irreversibly to the -receptor, and determined the role of the phosphatidylinositol 3-kinase (PI3K) pathway as a trigger or end effector of FIT-induced cardioprotection. Initially, male rats were administered FIT (10 姯kg) 10 min before hearts were subjected to 30 min of ischemia and 2 h of reperfusion followed by infarct size (IS) assessment. Acute FIT administration reduced IS when given before ischemia, 5 min before reperfusion, or 10 s after reperfusion compared with control. IS reduction also occurred following a single dose of FIT at 48, 72, 96, and 120 h after administration vs. control, with the maximum effect observed at 96 h. FIT-induced IS reduction at 96 h was completely abolished when the irreversible PI3K inhibitor wortmannin (15 姯kg) was given before FIT during the trigger phase; however, the effect was only partially abrogated when wortmannin was given 96 h later. These data suggest that FIT has a prolonged cardioprotective window greater than that of any previously described cardioprotective agent that requires PI3K primarily in the trigger phase but also partially, as a mediator or end effector
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Physiological Society
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefromH2744
dc.relation.ispartofpagetoH2749
dc.relation.ispartofjournalAmerican Journal of Physiology: Heart and Circulatory Physiology
dc.relation.ispartofvolume288
dc.rights.retentionY
dc.subject.fieldofresearchZoology
dc.subject.fieldofresearchMedical physiology
dc.subject.fieldofresearchCardiovascular medicine and haematology
dc.subject.fieldofresearchcode3109
dc.subject.fieldofresearchcode3208
dc.subject.fieldofresearchcode3201
dc.titleExtending the cardioprotective window using a novel δ-opioid agonist fentanyl isothiocyanate via the PI3-kinase pathway
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyrightSelf-archiving of the author-manuscript version is not yet supported by this journal. Please refer to the journal link for access to the definitive, published version or contact the author[s] for more information.
gro.date.issued2015-02-05T03:43:30Z
gro.hasfulltextNo Full Text
gro.griffith.authorPeart, Jason N.


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