β-Hemolysin-independent induction of apoptosis of macrophages infected with serotype III group B streptococcus
View/ Open
Author(s)
Ulett, GC
Bohnsack, JF
Armstrong, J
Adderson, EE
Griffith University Author(s)
Year published
2003
Metadata
Show full item recordAbstract
Group B streptococcus (GBS) induces apoptosis in macrophages. Growth conditions minimizing b-hemolysin expression, such as high glucose, reduce apoptosis. We constructed an isogenic mutant strain of GBS 874391 lacking the b-hemolysin structural gene cylE and investigated the role that bhemolysin plays in apoptosis of J774 macrophages. Viability of macrophages infected with wild-type or cylE GBS was similar and significantly less than that of macrophages infected with GBS grown in high-glucose media. Thus, apoptosis in GBS-infected macrophages is dependent not on b-hemolysin but on a factor coregulated with b-hemolysin by glucose.Group B streptococcus (GBS) induces apoptosis in macrophages. Growth conditions minimizing b-hemolysin expression, such as high glucose, reduce apoptosis. We constructed an isogenic mutant strain of GBS 874391 lacking the b-hemolysin structural gene cylE and investigated the role that bhemolysin plays in apoptosis of J774 macrophages. Viability of macrophages infected with wild-type or cylE GBS was similar and significantly less than that of macrophages infected with GBS grown in high-glucose media. Thus, apoptosis in GBS-infected macrophages is dependent not on b-hemolysin but on a factor coregulated with b-hemolysin by glucose.
View less >
View less >
Journal Title
Journal of Infectious Diseases
Volume
188
Issue
7
Publisher URI
Copyright Statement
© 2003 by University of Chicago Press. First published in Journal of Infectious Diseaseswith publishing partner University of Chicago. Use hypertext link to access the journal's webpage. The attached file is reproduced here in accordance with the copyright policy of the publisher.
Subject
Biological sciences
Biomedical and clinical sciences
Medical bacteriology