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dc.contributor.authorUlett, GC
dc.contributor.authorLabrooy, JT
dc.contributor.authorCurrie, BJ
dc.contributor.authorBarnes, JL
dc.contributor.authorKetheesan, N
dc.date.accessioned2017-05-03T15:29:27Z
dc.date.available2017-05-03T15:29:27Z
dc.date.issued2005
dc.date.modified2009-09-29T23:14:50Z
dc.identifier.issn1286-4579
dc.identifier.doi10.1016/j.micinf.2005.04.013
dc.identifier.urihttp://hdl.handle.net/10072/25847
dc.description.abstractBurkholderia pseudomallei, the etiological agent of melioidosis, causes significant mortality in endemic regions, but little is known regarding the immune mechanisms required for successful protective immunity. To establish a model of immunization that could be used to study this we screened a library of B. pseudomallei strains for immunogenicity in mice. BALB/c mice were immunized with test strains, and 2 weeks later were given a lethal challenge (LC) of virulent B. pseudomallei. Among 49 strains tested, a single strain, CL04, exhibited strong immunoprotective capacity. Interestingly, CL04 had been cultured from a patient with chronic colonization of B. pseudomallei, which is a rare phenomenon. Mice immunized with 0.1 נLD50 (5 נ10^3 CFU) of CL04 had significantly better survival and lower bacterial loads after LC compared to naﶥ controls. Dose-response analysis demonstrated more robust immunity after higher immunizing doses, and bacterial inactivation by gamma irradiation diminished the protective effect, indicating a requirement for viable organism for immunity. CL04-induced immunity was demonstrated both in B. pseudomallei-susceptible BALB/c and -resistant C57BL/6 mice. We investigated the gene profile of CL04-induced immunity by analyzing responses to immunization using cDNA microarray. Unique responses involving granulocyte macrophage colony stimulating factor (GM-CSF), the proapoptotic regulator Bad and cyclin-dependent kinase (CDK5) were detected in immunized mice, but these responses were absent in naﶥ-LC mice. Further, responses differed between mouse strains, indicating dependence on host genetic background. This model will be useful in identifying elements of the immune response required for successful adaptive immunity against B. pseudomallei.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier - Masson
dc.publisher.placeFrance
dc.publisher.urihttp://www.sciencedirect.com/science/journal/12864579
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1263
dc.relation.ispartofpageto1275
dc.relation.ispartofissue11
dc.relation.ispartofjournalMicrobes and Infection
dc.relation.ispartofvolume7
dc.rights.retentionY
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchMedical bacteriology
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode3204
dc.subject.fieldofresearchcode3207
dc.subject.fieldofresearchcode320701
dc.titleA Model of Immunity to Burkholderia pseudomallei: Unique Responses following Immunization and Acute Lethal Infection
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2005
gro.hasfulltextNo Full Text
gro.griffith.authorUlett, Glen C.


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