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dc.contributor.authorValle, Jaione
dc.contributor.authorMabbett, Amanda N
dc.contributor.authorUlett, Glen C
dc.contributor.authorToledo-Arana, Alejandro
dc.contributor.authorWecker, Karine
dc.contributor.authorTotsika, Makrina
dc.contributor.authorSchembri, Mark A
dc.contributor.authorGhigo, Jean-Marc
dc.contributor.authorBeloin, Christophe
dc.date.accessioned2017-09-05T02:52:28Z
dc.date.available2017-09-05T02:52:28Z
dc.date.issued2008
dc.date.modified2009-09-29T23:12:54Z
dc.identifier.issn0021-9193
dc.identifier.doi10.1128/JB.00122-08
dc.identifier.urihttp://hdl.handle.net/10072/25865
dc.description.abstractThe ability of Escherichia coli to colonize both intestinal and extraintestinal sites is driven by the presence of specific virulence factors, among which are the autotransporter (AT) proteins. Members of the trimeric AT adhesin family are important virulence factors for several gram-negative pathogens and mediate adherence to eukaryotic cells and extracellular matrix (ECM) proteins. In this study, we characterized a new trimeric AT adhesin (UpaG) from uropathogenic E. coli (UPEC). Molecular analysis of UpaG revealed that it is translocated to the cell surface and adopts a multimeric conformation. We demonstrated that UpaG is able to promote cell aggregation and biofilm formation on abiotic surfaces in CFT073 and various UPEC strains. In addition, UpaG expression resulted in the adhesion of CFT073 to human bladder epithelial cells, with specific affinity to fibronectin and laminin. Prevalence analysis revealed that upaG is strongly associated with E. coli strains from the B2 and D phylogenetic groups, while deletion of upaG had no significant effect on the ability of CFT073 to colonize the mouse urinary tract. Thus, UpaG is a novel trimeric AT adhesin from E. coli that mediates aggregation, biofilm formation, and adhesion to various ECM proteins.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.publisher.placeUSA
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom4147
dc.relation.ispartofpageto4161
dc.relation.ispartofissue12
dc.relation.ispartofjournalJournal of Bacteriology
dc.relation.ispartofvolume190
dc.rights.retentionY
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchAgricultural, veterinary and food sciences
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchMedical bacteriology
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode30
dc.subject.fieldofresearchcode32
dc.subject.fieldofresearchcode320701
dc.titleUpaG, a new member of the trimeric autotransporter family of adhesins in uropathogenic Escherichia coli
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2008 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.date.issued2008
gro.hasfulltextFull Text
gro.griffith.authorUlett, Glen C.


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