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  • Comparison of the brine shrimp nauplii bioassay and the ToxScreen-II Test for the detection of toxicity associated with Aloe vera (Aloe barbadensis Miller) leaf extract

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    Author(s)
    Cock, Ian
    Ruebhart, D.
    Griffith University Author(s)
    Cock, Ian E.
    Year published
    2009
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    Abstract
    This study assessed the performance of a bioluminescent bacterial assay, the ToxScreen-II Test (Photobacterium leiognathi), in comparison to the brine shrimp (Artemia franciscana) nauplii lethality assay for the detection of toxicity associated with Aloe vera leaf gel extract and the organophosphate pesticide Mevinphos. In both bioassays, clear dose-response relationships were demonstrated for these two test substances. Sensitivity to the Aloe vera leaf extract was similar for both bioassays, with 1 h IC50 of 2 994 )g/ml in the the ToxScreen-II Test and 2 h LC50 of 3 940 )g/ml in the brine shrimp nauplii bioassay. In contrast, ...
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    This study assessed the performance of a bioluminescent bacterial assay, the ToxScreen-II Test (Photobacterium leiognathi), in comparison to the brine shrimp (Artemia franciscana) nauplii lethality assay for the detection of toxicity associated with Aloe vera leaf gel extract and the organophosphate pesticide Mevinphos. In both bioassays, clear dose-response relationships were demonstrated for these two test substances. Sensitivity to the Aloe vera leaf extract was similar for both bioassays, with 1 h IC50 of 2 994 )g/ml in the the ToxScreen-II Test and 2 h LC50 of 3 940 )g/ml in the brine shrimp nauplii bioassay. In contrast, the ToxScreen-II Test was approximately 10 fold more sensitivity towards Mevinphos (1 h IC50 113 )g/ml) than the brine shrimp nauplii test (24 h LC50 1 316). While the ToxScreen-II Test was developed as a tool primarily for the detection of anthropogenic chemicals in water, this study showed that it can also be used for the screening of bioactivity in a complex plant extract. The advantages of this bioluminescent bacterial assay, over the traditionally used brine shrimp test, included a quicker turn around time, reduced preparation of the test organism and other materials, and the acquisition of test results within 1 hour.
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    Journal Title
    Pharmacognosy Research
    Volume
    1
    Issue
    2
    Publisher URI
    http://www.phcogmag.com/
    Copyright Statement
    © 2009 Phcog.net. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
    Subject
    Biological Sciences not elsewhere classified
    Plant Biology
    Complementary and Alternative Medicine
    Pharmacology and Pharmaceutical Sciences
    Publication URI
    http://hdl.handle.net/10072/26552
    Collection
    • Journal articles

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