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dc.contributor.authorB. Ashman, Robert
dc.date.accessioned2017-05-03T16:58:38Z
dc.date.available2017-05-03T16:58:38Z
dc.date.issued2008
dc.date.modified2013-05-29T00:02:07Z
dc.identifier.issn10939946
dc.identifier.doi10.2741/2929
dc.identifier.urihttp://hdl.handle.net/10072/26730
dc.description.abstractIn this study, the low molecular weight lypophilic metabolites of C.albicans and C. dubliniensis strains produced in a synthetic medium with the addition of fetal calf serum were identified using LC/MS and MS/MS technique and quantified. All strains investigated produce a metabolite with a UV spectra maximum at 224 and 279 nm and minimum at 243 nm. Following comparison with ESI, MS/MS spectral data of a reference compound, the metabolite was identified as 3-indoleethanol (tryptophol). The concentration of extracellular tryptophol in the biosynthesis of C. albicans and C. dubliniensis ranged from 2.45 姯mL to 191 姯mL, respectively. Contrary to previously published data, gliotoxin or gliotoxin-like compounds were not detected, and all investigated C. albicans and C. dubliniensis strains have the same metabolite profile. Cytotoxic effects of tryptophol and 3-indolelactic acid (precursor of tryptophol biosynthesis) were cell-line-dependent. The EC50 of tryptophol ranged between 2 and 7 mM, with the EC50 of 3-indolelactic acid approximately double (between 4 and 8 mM). Tryptophol exhibited cell-type dependent cytotoxicity in relatively high concentrations, with domination of apoptosis.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherFrontiers in Bioscience
dc.publisher.placeUnited States
dc.publisher.urihttp://www.bioscience.org/
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom3334
dc.relation.ispartofpageto3351
dc.relation.ispartofjournalFrontiers in Bioscience
dc.relation.ispartofvolume13
dc.rights.retentionY
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchOncology and Carcinogenesis
dc.subject.fieldofresearchMedical Physiology
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1112
dc.subject.fieldofresearchcode1116
dc.titleProtective and pathologic immune responses against Candida albicans infection
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyrightSelf-archiving of the author-manuscript version is not yet supported by this journal. Please refer to the journal link for access to the definitive, published version or contact the author for more information.
gro.date.issued2008
gro.hasfulltextNo Full Text
gro.griffith.authorAshman, Robert B.


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