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dc.contributor.authorPiens, Kathleenen_US
dc.contributor.authorHenriksson, Anna-Mariaen_US
dc.contributor.authorGullfot, Fredrikaen_US
dc.contributor.authorLopez, Marieen_US
dc.contributor.authorFauré, Régisen_US
dc.contributor.authorM. Ibatullin, Fariden_US
dc.contributor.authorT. Teeri, Tuulaen_US
dc.contributor.authorDriguez, Huguesen_US
dc.contributor.authorBrumer, Harryen_US
dc.contributor.editorDr. Vikki Allenen_US
dc.date.accessioned2017-05-03T16:58:23Z
dc.date.available2017-05-03T16:58:23Z
dc.date.issued2007en_US
dc.date.modified2009-12-05T05:16:25Z
dc.identifier.issn1477-0520en_US
dc.identifier.doi10.1039/b714570een_AU
dc.identifier.urihttp://hdl.handle.net/10072/27096
dc.description.abstractGlycosynthases are active-site mutants of glycoside hydrolases that catalyse glycosyl transfer using suitable activated donor substrates without competing product hydrolysis (S. M. Hancock, M. D. Vaughan and S. G. Withers, Curr. Opin. Chem. Biol., 2006, 10, 509-519). Site-directed mutagenesis of the catalytic nucleophile, Glu-85, of a Populus tremula x tremuloides xyloglucan endo-transglycosylase (PttXET16-34, EC 2.4.1.207) into alanine, glycine, and serine yielded enzymes with glycosynthase activity. Product analysis indicated that PttXET16-34 E85A in particular was able to catalyse regio- and stereospecific homo- and hetero-condensations of -xylogluco-oligosaccharyl fluoride donors XXXGF and XLLGF to produce xyloglucans with regular sidechain substitution patterns. This substrate promiscuity contrasts that of the Humicola insolens Cel7B E197A glycosynthase, which was not able to polymerise the di-galactosylated substrate XLLGF. The production of the PttXET16-34 E85A xyloglucosynthase thus expands the repertoire of glycosynthases to include those capable of synthesising structurally homogenenous xyloglucans for applications.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherRoyal Society of Chemistryen_US
dc.publisher.placeUnited Kingdomen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom3971en_US
dc.relation.ispartofpageto3978en_US
dc.relation.ispartofjournalOrganic & Biomolecular Chemistryen_US
dc.relation.ispartofvolume5en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchOrganic Chemical Synthesisen_US
dc.subject.fieldofresearchcode030503en_US
dc.titleGlycosynthase activity of hybrid aspen xyloglucan endo-transglycosylase PttXET16-34 nucleophile mutantsen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2007
gro.hasfulltextNo Full Text


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