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dc.contributor.authorHouston, Katrina M
dc.contributor.authorSutharsan, Ratneswary
dc.contributor.authorSteiger, Christina N
dc.contributor.authorSchachter, Harry
dc.contributor.authorHarnett, William
dc.contributor.editorA P Waters, C E Clayton
dc.date.accessioned2017-05-03T16:58:24Z
dc.date.available2017-05-03T16:58:24Z
dc.date.issued2008
dc.date.modified2009-12-05T05:16:32Z
dc.identifier.issn0166-6851
dc.identifier.doi10.1016/j.molbiopara.2007.08.009
dc.identifier.urihttp://hdl.handle.net/10072/27097
dc.description.abstractAn unusual feature of nematodes is the covalent attachment of immunomodulatory phosphorylcholine (PC) moieties to N-type glycans. Our previous work on the filarial nematode glycoprotein ES-62 has enabled us to predict the identity of enzymes necessary for PC-N-glycan biosynthesis. Here, we addressed these predictions using gene knockout technology applied to C. elegans and present two pieces of confirmatory data. Employing a triple null mutant worm lacking all three genes that encode active UDP-N-acetyl-d-glucosamine: a-3-d-mannoside ߱, 2-N-acetylglucosaminyltransferase I (GnT I) we have confirmed our earlier prediction that a crucial step in the generation of the substrate for PC transfer is addition of terminal GlcNAc to the a1-3-linked mannose residue of the glycan by GnT I. Second, by silencing genes responsible for expressing enzymes of the Kennedy pathway of phosphatidylcholine biosynthesis by RNA interference (RNAi), we have confirmed our belief for a role for diacylglycerol: choline phosphotransferase (CPT) in PC-N-glycan biosynthesis.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom88
dc.relation.ispartofpageto91
dc.relation.ispartofissue1
dc.relation.ispartofjournalMolecular and Biochemical Parasitology
dc.relation.ispartofvolume157
dc.rights.retentionY
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchAgricultural and Veterinary Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode07
dc.subject.fieldofresearchcode11
dc.titleGene inactivation confirms the identity of enzymes involved in nematode phosphorylcholine-N-glycan synthesis
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2007
gro.hasfulltextNo Full Text
gro.griffith.authorSutharsan, Ratneswary


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