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dc.contributor.authorCarninci, Piero
dc.contributor.authorWaki, Kazunori
dc.contributor.authorShiraki, Toshiyuki
dc.contributor.authorKonno, Hideaki
dc.contributor.authorShibata, Kazuhiro
dc.contributor.authorItoh, Masayoshi
dc.contributor.authorAizawa, Katsunori
dc.contributor.authorArakawa, Takahiro
dc.contributor.authorIshii, Yoshiyuki
dc.contributor.authorSasaki, Daisuke
dc.contributor.authorBono, Hidemasa
dc.contributor.authorKondo, Shinji
dc.contributor.authorSugahara, Yuichi
dc.contributor.authorSaito, Rintaro
dc.contributor.authorOsato, Naoki
dc.contributor.authorFukuda, Shiro
dc.contributor.authorSato, Kenjiro
dc.contributor.authorWatahiki, Akira
dc.contributor.authorHirozane-Kishikawa, Tomoko
dc.contributor.authorNakamura, Mari
dc.contributor.authorShibata, Yuko
dc.contributor.authorYasunishi, Ayako
dc.contributor.authorKikuchi, Noriko
dc.contributor.authorYoshiki, Atsushi
dc.contributor.authorKusakabe, Moriaki
dc.contributor.authorGustincich, Stefano
dc.contributor.authorBeisel, Kirk
dc.contributor.authorPavan, William
dc.contributor.authorAidinis, Vassilis
dc.contributor.authorNakagawara, Akira
dc.contributor.authorHeld, William A.
dc.contributor.authorIwata, Hiroo
dc.contributor.authorKono, Tomohiro
dc.contributor.authorNakauchi, Hiromitsu
dc.contributor.authorLyons, Paul
dc.contributor.authorWells, C.
dc.contributor.authorHume, David A.
dc.contributor.authorFagiolini, Michela
dc.contributor.authoret al.
dc.date.accessioned2017-09-06T03:06:11Z
dc.date.available2017-09-06T03:06:11Z
dc.date.issued2003
dc.identifier.issn10889051en_US
dc.identifier.doi10.1101/gr.1119703en_US
dc.identifier.urihttp://hdl.handle.net/10072/27150
dc.description.abstractWe report the construction of the mouse full-length cDNA encyclopedia,th e most extensive view of a complex transcriptome,on the basis of preparing and sequencing 246 libraries. Before cloning,cDNAs were enriched in full-length by Cap-Trapper,and in most cases,aggressively subtracted/normalized. We have produced 1,442,236 successful 3'-end sequences clustered into 171,144 groups, from which 60,770 clones were fully sequenced cDNAs annotated in the FANTOM-2 annotation. We have also produced 547,149 5' end reads,which clustered into 124,258 groups. Altogether, these cDNAs were further grouped in 70,000 transcriptional units (TU),which represent the best coverage of a transcriptome so far. By monitoring the extent of normalization/subtraction, we define the tentative equivalent coverage (TEC),which was estimated to be equivalent to >12,000,000 ESTs derived from standard libraries. High coverage explains discrepancies between the very large numbers of clusters (and TUs) of this project,which also include non-protein-coding RNAs,an d the lower gene number estimation of genome annotations. Altogether,5'-end clusters identify regions that are potential promoters for 8637 known genes and 5'-end clusters suggest the presence of almost 63,000 transcriptional starting points. An estimate of the frequency of polyadenylation signals suggests that at least half of the singletons in the EST set represent real mRNAs. Clones accounting for about half of the predicted TUs await further sequencing. The continued high-discovery rate suggests that the task of transcriptome discovery is not yet complete.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherCold Spring Harbor Laboratory Pressen_US
dc.publisher.placeUSAen_US
dc.relation.ispartofpagefrom1273en_US
dc.relation.ispartofpageto1289en_US
dc.relation.ispartofissue6ben_US
dc.relation.ispartofjournalGenome Researchen_US
dc.relation.ispartofvolume13en_US
dc.subject.fieldofresearchGenetics not elsewhere classifieden_US
dc.subject.fieldofresearchcode060499en_US
dc.titleTargeting a Complex Transcriptome: The Construction of the Mouse Full-Length cDNA Encyclopediaen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
dc.description.versionPublisheden_US
gro.rights.copyright© 2003 Cold Spring Harbor Laboratory Press. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.en_US
gro.date.issued2014-10-10T01:56:28Z
gro.hasfulltextFull Text
gro.griffith.authorWells, Christine


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