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dc.contributor.authorWoodruff, Maria Ann
dc.contributor.authorRath, Subha Narayan
dc.contributor.authorSusanto, Evelyn
dc.contributor.authorHaupt, Larisa M
dc.contributor.authorHutmacher, Dietmar W
dc.contributor.authorNurcombe, Victor
dc.contributor.authorCool, Simon M
dc.date.accessioned2017-05-03T16:58:45Z
dc.date.available2017-05-03T16:58:45Z
dc.date.issued2007
dc.date.modified2009-12-07T03:41:02Z
dc.identifier.issn1567-2379
dc.identifier.doi10.1007/s10735-007-9137-y
dc.identifier.urihttp://hdl.handle.net/10072/27176
dc.description.abstractThis paper explores the potential therapeutic role of the naturally occurring sugar heparan sulfate (HS) for the augmentation of bone repair. Scaffolds comprising fibrin glue loaded with 5 姠of embryonically derived HS were assessed, firstly as a release-reservoir, and secondly as a scaffold to stimulate bone regeneration in a critical size rat cranial defect. We show HS-loaded scaffolds have a uniform distribution of HS, which was readily released with a typical burst phase, quickly followed by a prolonged delivery lasting several days. Importantly, the released HS contributed to improved wound healing over a 3-month period as determined by microcomputed tomography (僔) scanning, histology, histomorphometry, and PCR for osteogenic markers. In all cases, only minimal healing was observed after 1 and 3 months in the absence of HS. In contrast, marked healing was observed by 3 months following HS treatment, with nearly full closure of the defect site. PCR analysis showed significant increases in the gene expression of the osteogenic markers Runx2, alkaline phosphatase, and osteopontin in the heparin sulfate group compared with controls. These results further emphasize the important role HS plays in augmenting wound healing, and its successful delivery in a hydrogel provides a novel alternative to autologous bone graft and growth factor-based therapies.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer
dc.publisher.placeNetherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom425
dc.relation.ispartofpageto433
dc.relation.ispartofissue5
dc.relation.ispartofjournalJournal of Molecular Histology
dc.relation.ispartofvolume38
dc.rights.retentionY
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classified
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchCardiorespiratory Medicine and Haematology
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchcode060199
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1102
dc.subject.fieldofresearchcode1103
dc.titleSustained release and osteogenic potential of heparan sulfate-doped fibrin glue scaffolds within a rat cranial model
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2007
gro.hasfulltextNo Full Text
gro.griffith.authorHaupt, Larisa
gro.griffith.authorHutmacher, Dietmar W.


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