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  • Temporal and functional changes in glycosaminoglycan expression during osteogenesis

    Author(s)
    Nurcombe, Victor
    Goh, Fuqi Jack
    M. Haupt, Larisa
    Murali, Sadasivam
    M. Cool, Simon
    Griffith University Author(s)
    Haupt, Larisa
    Year published
    2007
    Metadata
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    Abstract
    Heparan sulfate proteoglycans (HSPGs) are complex and labile macromolecular moieties on the surfaces of cells that control the activities of a range of extracellular proteins, particularly those driving growth and regeneration. Here, we examine the biosynthesis of heparan sulfate (HS) sugars produced by cultured MC3T3-E1 mouse calvarial pre-osteoblast cells in order to explore the idea that changes in HS activity in turn drive phenotypic development during osteogenesis. Cells grown for 5 days under proliferating conditions were compared to cells grown for 20 days under mineralizing conditions with respect to their phenotype, ...
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    Heparan sulfate proteoglycans (HSPGs) are complex and labile macromolecular moieties on the surfaces of cells that control the activities of a range of extracellular proteins, particularly those driving growth and regeneration. Here, we examine the biosynthesis of heparan sulfate (HS) sugars produced by cultured MC3T3-E1 mouse calvarial pre-osteoblast cells in order to explore the idea that changes in HS activity in turn drive phenotypic development during osteogenesis. Cells grown for 5 days under proliferating conditions were compared to cells grown for 20 days under mineralizing conditions with respect to their phenotype, the forms of HS core protein produced, and their HS sulfotransferase biosynthetic enzyme levels. RQ-PCR data was supported by the results from the purification of day 5 and day 20 HS forms by anionic exchange chromatography. The data show that cells in active growth phases produce more complex forms of sugar than cells that have become relatively quiescent during active mineralization, and that these in turn can differentially influence rates of cell growth when added exogenously back to preosteoblasts.
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    Journal Title
    Journal of Molecular Histology
    Volume
    38
    Issue
    5
    DOI
    https://doi.org/10.1007/s10735-007-9123-4
    Subject
    Biochemistry and Cell Biology not elsewhere classified
    Biochemistry and Cell Biology
    Cardiorespiratory Medicine and Haematology
    Clinical Sciences
    Publication URI
    http://hdl.handle.net/10072/27184
    Collection
    • Journal articles

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