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dc.contributor.authorWang, Penghuaen_US
dc.contributor.authorZhang, Yongen_US
dc.contributor.authorLi, Hongzheen_US
dc.contributor.authorChieu, Hai Keeen_US
dc.contributor.authorMunn, Alanen_US
dc.contributor.authorYang, Hongyuanen_US
dc.contributor.editorPernille Rorthen_US
dc.date.accessioned2017-04-24T14:55:34Z
dc.date.available2017-04-24T14:55:34Z
dc.date.issued2005en_US
dc.date.modified2009-12-07T07:55:49Z
dc.identifier.issn14602075en_US
dc.identifier.doi10.1038/sj.emboj.7600764en_AU
dc.identifier.urihttp://hdl.handle.net/10072/27209
dc.description.abstractThe yeast genome encodes seven oxysterol binding protein homologs, Osh1p-Osh7p, which have been implicated in regulating intracellular lipid and vesicular transport. Here, we show that both Osh6p and Osh7p interact with Vps4p, a member of the AAA (ATPases associated with a variety of cellular activities) family. The coiled-coil domain of Osh7p was found to interact with Vps4p in a yeast two-hybrid screen and the interaction between Osh7p and Vps4p appears to be regulated by ergosterol. Deletion of VPS4 induced a dramatic increase in the membrane-associated pools of Osh6p and Osh7p and also caused a decrease in sterol esterification, which was suppressed by overexpression of OSH7. Lastly, overexpression of the coiled-coil domain of Osh7p (Osh7pCC) resulted in a multivesicular body sorting defect, suggesting a dominant negative role of Osh7pCC possibly through inhibiting Vps4p function. Our data suggest that a common mechanism may exist for AAA proteins to regulate the membrane association of yeast OSBP proteins and that these two protein families may function together to control subcellular lipid transport.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherNature Publishing Groupen_US
dc.publisher.placeUnited Kingdomen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom2989en_US
dc.relation.ispartofpageto2999en_US
dc.relation.ispartofjournalEMBO journalen_US
dc.relation.ispartofvolume24en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchcode270199en_US
dc.titleAAA ATPases regulate membrane association of yeast oxysterol binding proteins and sterol metabolismen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2005
gro.hasfulltextNo Full Text


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