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dc.contributor.authorWang, Penghua
dc.contributor.authorZhang, Yong
dc.contributor.authorLi, Hongzhe
dc.contributor.authorChieu, Hai Kee
dc.contributor.authorMunn, Alan
dc.contributor.authorYang, Hongyuan
dc.contributor.editorPernille Rorth
dc.date.accessioned2017-05-03T16:58:43Z
dc.date.available2017-05-03T16:58:43Z
dc.date.issued2005
dc.date.modified2009-12-07T07:55:49Z
dc.identifier.issn14602075
dc.identifier.doi10.1038/sj.emboj.7600764
dc.identifier.urihttp://hdl.handle.net/10072/27209
dc.description.abstractThe yeast genome encodes seven oxysterol binding protein homologs, Osh1p-Osh7p, which have been implicated in regulating intracellular lipid and vesicular transport. Here, we show that both Osh6p and Osh7p interact with Vps4p, a member of the AAA (ATPases associated with a variety of cellular activities) family. The coiled-coil domain of Osh7p was found to interact with Vps4p in a yeast two-hybrid screen and the interaction between Osh7p and Vps4p appears to be regulated by ergosterol. Deletion of VPS4 induced a dramatic increase in the membrane-associated pools of Osh6p and Osh7p and also caused a decrease in sterol esterification, which was suppressed by overexpression of OSH7. Lastly, overexpression of the coiled-coil domain of Osh7p (Osh7pCC) resulted in a multivesicular body sorting defect, suggesting a dominant negative role of Osh7pCC possibly through inhibiting Vps4p function. Our data suggest that a common mechanism may exist for AAA proteins to regulate the membrane association of yeast OSBP proteins and that these two protein families may function together to control subcellular lipid transport.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom2989
dc.relation.ispartofpageto2999
dc.relation.ispartofjournalEMBO journal
dc.relation.ispartofvolume24
dc.rights.retentionY
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchInformation and Computing Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode08
dc.subject.fieldofresearchcode11
dc.titleAAA ATPases regulate membrane association of yeast oxysterol binding proteins and sterol metabolism
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2005
gro.hasfulltextNo Full Text
gro.griffith.authorMunn, Alan L.


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