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dc.contributor.authorRen, Gang
dc.contributor.authorWang, Juan
dc.contributor.authorBrinkworth, Ross
dc.contributor.authorWinsor, Barbara
dc.contributor.authorKobe, Bostjan
dc.contributor.authorL. Munn, Alan
dc.date.accessioned2017-05-03T16:58:44Z
dc.date.available2017-05-03T16:58:44Z
dc.date.issued2005
dc.date.modified2009-12-07T07:55:52Z
dc.identifier.issn13989219
dc.identifier.doi10.1111/j.1600-0854.2005.00300.x
dc.identifier.urihttp://hdl.handle.net/10072/27210
dc.description.abstractIn budding yeast, partitioning of the cytoplasm during cytokinesis can proceed via a pathway dependent on the contractile actomyosin ring, as in other eukaryotes, or alternatively via a septum deposition pathway dependent on an SH3 domain protein, Hof1/Cyk2 (the yeast PSTPIP1 ortholog). In dividing yeast cells, Hof1 forms a ring at the bud neck distinct from the actomyosin ring, and this zone is active in septum deposition. We previously showed the yeast Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) ortholog, verprolin/Vrp1/End5, interacts with Hof1 and facilitates Hof1 recruitment to the bud neck. A Vrp1 fragment unable to interact with yeast WASP (Las17/Bee1), localize to the actin cytoskeleton or function in polarization of the cortical actin cytoskeleton nevertheless retains function in Hof1 recruitment and cytokinesis. Here, we show the ability of this Vrp1 fragment to bind the Hof1 SH3 domain via its Hof one trap (HOT) domain is critical for cytokinesis. The Vrp1 HOT domain consists of three tandem proline-rich motifs flanked by serines. Unexpectedly, the Hof1 SH3 domain itself is not required for cytokinesis and indeed appears to negatively regulate cytokinesis. The Vrp1 HOT domain promotes cytokinesis by binding to the Hof1 SH3 domain and counteracting its inhibitory effect.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBlackwell Munksgaard
dc.publisher.placeDenmark
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom575
dc.relation.ispartofpageto593
dc.relation.ispartofissue7
dc.relation.ispartofjournalTraffic
dc.relation.ispartofvolume6
dc.rights.retentionY
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1108
dc.titleVerprolin Cytokinesis Function Mediated by the Hof One Trap Domain
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2005
gro.hasfulltextNo Full Text
gro.griffith.authorMunn, Alan L.


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