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dc.contributor.authorBartlett, Selena E.
dc.contributor.authorJ. Reynolds, Anna
dc.contributor.authorII, Michael Weible
dc.contributor.authorA. Hendry, Ian
dc.date.accessioned2017-05-03T16:58:32Z
dc.date.available2017-05-03T16:58:32Z
dc.date.issued2002
dc.date.modified2009-12-09T07:41:58Z
dc.identifier.issn01616412
dc.identifier.doi10.1002/jnr.10201
dc.identifier.urihttp://hdl.handle.net/10072/27250
dc.description.abstractPhosphatidylinositol 3-kinase (PI3-kinase) and phosphatidylinositol 4-kinase (PI4-kinase) enzymes are an important family of signaling molecules that have been implicated in the regulation of intracellular vesicle trafficking. It has previously been shown that PI3-kinase and PI4-kinase enzymes regulate neuronal survival and the retrograde axonal transport of nerve growth factor in sympathetic and sensory neurons. We have extended these studies to examine the role these enzymes play in the regulation of the retrograde axonal transport of neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) in sympathetic and sensory neurons in vivo. Wortmannin (0.1 nmol/eye), a PI3-kinase and PI4-kinase antagonist, reduced the amount of 125I-NT-3 retrograde transport in sympathetic neurons by approximately 50% and 125I-NT-4 in sympathetic neurons by approximately 40% and sensory neurons by approximately 20%. The PI3-kinase antagonist LY294002 (100 nmol/eye) reduced the retrograde axonal transport of 125I-NT-4 in sympathetic and sensory neurons, and 125I-NT-3 in sympathetic neurons. Phenylarsine oxide (PAO), a PI4-kinase antagonist, significantly inhibited 125I-NT-4 retrograde axonal transport in sympathetic and sensory neurons. These results show that wortmannin-sensitive PI3-kinases and PI4-kinases may be involved in NT-3 and NT-4 retrograde axonal transport. The retrograde axonal transport of neurotrophic factors in sympathetic and sensory neurons in vivo appears to depend upon the activation of different receptors and second messenger cascades at the nerve terminal.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherJohn Wiley
dc.publisher.placeUS
dc.relation.ispartofpagefrom169
dc.relation.ispartofpageto175
dc.relation.ispartofissue2
dc.relation.ispartofjournalJournal of Neuroscience Research
dc.relation.ispartofvolume68
dc.subject.fieldofresearchNeurosciences not elsewhere classified
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchNeurosciences
dc.subject.fieldofresearchcode110999
dc.subject.fieldofresearchcode1103
dc.subject.fieldofresearchcode1109
dc.titlePhosphatidylinositol kinase enzymes regulate the retrograde axonal transport of NT-3 and NT-4 in sympathetic and sensory neurons
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2002
gro.hasfulltextNo Full Text
gro.griffith.authorWeible, Michael W.


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