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dc.contributor.authorSmith, S
dc.contributor.authorMorin, PA
dc.date.accessioned2017-05-03T16:59:50Z
dc.date.available2017-05-03T16:59:50Z
dc.date.issued2005
dc.date.modified2011-05-06T06:43:56Z
dc.identifier.issn0022-1198
dc.identifier.urihttp://hdl.handle.net/10072/27412
dc.description.abstractDNA extraction from trace samples or noninvasively collected samples often results in the recovery of low concentration solutions of DNA that are prone to DNA degradation or other loss. Because of the difficulty in obtaining such samples, and their potentially high value in wildlife and forensic studies, it is critical that optimal methods are employed for their long-term storage. We assessed the amplification yield of samples kept under different storage conditions with the addition of potential preserving agents. We stored dilutions of known concentration human placental DNA, and gorilla fecal DNA, under four conditions (+4ì -20ì -80ì dry at room temperature), and with three additives (Tris EDTA (TE) buffer, Hind III digested Lambda DNA, trehalose). The effectiveness of the treatment methods was tested at regular intervals using qPCR to assess the quantity of amplifiable DNA, and a PCR assay of a larger 757 bp fragment to evaluate the quality of that remaining DNA. The highest quantity of DNA remained in samples stored at -80ì regardless of storage additives, and those dried at room temperature in the presence of trehalose. Surprisingly, DNA quality was best preserved in the presence of trehalose, either dried or at -80û significant quality loss occurred with -20àand +4àstorage.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing, Inc.
dc.publisher.placeUnited States
dc.publisher.urihttp://www.astm.org/DIGITAL_LIBRARY/JOURNALS/FORENSIC/index.html
dc.publisher.urihttp://www.astm.org/DIGITAL_LIBRARY/JOURNALS/FORENSIC/PAGES/JFS2004411.htm
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1101
dc.relation.ispartofpageto1108
dc.relation.ispartofissue5
dc.relation.ispartofjournalJournal of Forensic Sciences
dc.relation.ispartofvolume50
dc.rights.retentionY
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchOther Biological Sciences
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchcode0399
dc.subject.fieldofresearchcode0699
dc.subject.fieldofresearchcode1103
dc.titleOptimal Storage Conditions for Highly Dilute DNA Samples: A Role for Trehalose as a Preserving Agent
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Sciences, Griffith School of Environment
gro.date.issued2005
gro.hasfulltextNo Full Text
gro.griffith.authorSmith, Steven J.


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    Contains articles published by Griffith authors in scholarly journals.

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