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dc.contributor.authorBulmer, AC
dc.contributor.authorRied, K
dc.contributor.authorCoombes, JS
dc.contributor.authorBlanchfield, JT
dc.contributor.authorToth, I
dc.contributor.authorWagner, KH
dc.date.accessioned2017-05-03T16:59:43Z
dc.date.available2017-05-03T16:59:43Z
dc.date.issued2007
dc.date.modified2009-12-14T03:14:56Z
dc.identifier.issn1383-5718
dc.identifier.doi10.1016/j.mrgentox.2007.01.008
dc.identifier.urihttp://hdl.handle.net/10072/27433
dc.description.abstractThe aim of this study was to explore the potential pro- and anti-mutagenic effects of endogenous bile pigments unconjugated bilirubin (BR), biliverdin (BV) and a synthetic, water soluble conjugate, bilirubin ditaurate (BRT) in the Ames Salmonella test. The bile pigments were tested over a wide concentration range (0.01-2 孯l/plate) in the presence of three bacterial strains (TA98, TA100, TA102). A variety of mutagens including benzo[a]pyrene (B[a]P), 2,4,7 trinitrofluorenone (TNFone), 2-aminofluorene (2-AF), sodium azide (NaN3) and tertiary-butyl hydroperoxide (t-BuOOH), were used to promote the formation of mutant revertants. Tests were conducted with (B[a]P, 2-AF, t-BuOOH) and without (TNFone, NaN3, t-BuOOH) metabolic activation incorporating the addition of the microsomal liver preparation, S9. The bile pigments alone did not induce mutagenicity in any of the strains tested (p > 0.05). Anti-mutagenic effects of the bile pigments were observed in the presence of all mutagens except for NaN3 and the anti-mutagenic effects appeared independent of the strain tested. For TNFone induced genotoxicity, the order of effectiveness was BR = BRT > BV. However, the order was BV = BRT = BR for 2-AF. Antioxidant testing in the TA102 strain revealed bile pigments could effectively inhibit the genotoxic effect of t-BuOOH induced oxidative stress. The apparent antioxidant and anti-mutagenic behaviour of bile pigments further suggests their presence in biological systems is of possible physiological importance.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.publisher.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/522820/description#description
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom122
dc.relation.ispartofpageto132
dc.relation.ispartofissue2
dc.relation.ispartofjournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
dc.relation.ispartofvolume629
dc.rights.retentionY
dc.subject.fieldofresearchMedical and Health Sciences not elsewhere classified
dc.subject.fieldofresearchEnvironmental Biotechnology
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode119999
dc.subject.fieldofresearchcode1002
dc.subject.fieldofresearchcode1115
dc.titleThe anti-mutagenic and antioxidant effects of bile pigments in the Ames Salmonella test
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2007
gro.hasfulltextNo Full Text
gro.griffith.authorBulmer, Andrew C.


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