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  • p38-MAPK and PKB/Akt, possible role players in red palm oil-induced protection of the isolated perfused rat heart?

    Author(s)
    Engelbrecht, AM
    Esterhuyse, J
    Du Toit, EF
    Lochner, A
    Van Rooyen, J
    Griffith University Author(s)
    Du Toit, Eugene
    Year published
    2006
    Metadata
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    Abstract
    It has been shown that dietary red palm oil (RPO) supplementation improves reperfusion function. However, no exact protective cellular mechanisms have been established. To determine a potential mechanism for functional improvement, we examined the regulation of both mitogen-activated protein kinases (MAPKs) and PKB/Akt in the presence and absence of dietary RPO supplementation in ischemia/reperfusion-induced injury. Wistar rats were fed a control diet or control diet plus 7 g RPO/kg diet for 6 weeks. Hearts were excised and mounted on an isolated working heart perfusion apparatus. Cardiac function was measured before and ...
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    It has been shown that dietary red palm oil (RPO) supplementation improves reperfusion function. However, no exact protective cellular mechanisms have been established. To determine a potential mechanism for functional improvement, we examined the regulation of both mitogen-activated protein kinases (MAPKs) and PKB/Akt in the presence and absence of dietary RPO supplementation in ischemia/reperfusion-induced injury. Wistar rats were fed a control diet or control diet plus 7 g RPO/kg diet for 6 weeks. Hearts were excised and mounted on an isolated working heart perfusion apparatus. Cardiac function was measured before and after hearts were subjected to 25 min of total global ischemia. Hearts subjected to the same conditions were freeze clamped and used to characterize the degree of phosphorylation of extracellular signal-regulated kinase, p38, c-Jun NH2-terminal protein kinase (JNK) and PKB/Akt. Dietary RPO supplementation significantly improved aortic output recovery (72.1ᳮ2% vs. 54.0ᳮ2%, P<.05). This improved aortic output recovery was associated with significant increases in p38 and PKB/Akt phosphorylation during reperfusion when compared with control hearts. Furthermore, a significant decrease in JNK phosphorylation and attenuation of poly(ADP-ribose) polymerase cleavage occurred in the RPO-supplemented group during reperfusion. Our results suggest that dietary RPO supplementation caused differential phosphorylation of the MAPKs and PKB/Akt during ischemia/reperfusion-induced injury. These changes in phosphorylation were associated with improved functional recovery and reduced cleavage of an apoptotic marker, arguing that dietary RPO supplementation may confer protection via the MAPK and PKB/Akt signaling pathways during ischemia/reperfusion-induced injury.
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    Journal Title
    Journal of Nutritional Biochemistry
    Volume
    17
    Issue
    4
    Publisher URI
    http://www.sciencedirect.com/science/journal/09552863
    DOI
    https://doi.org/10.1016/j.jnutbio.2005.05.001
    Subject
    Biochemistry and cell biology
    Food sciences
    Cardiology (incl. cardiovascular diseases)
    Nutrition and dietetics
    Publication URI
    http://hdl.handle.net/10072/27480
    Collection
    • Journal articles

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