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dc.contributor.authorUlett, Glen C
dc.contributor.authorMabbett, Amanda N
dc.contributor.authorFung, Khe C
dc.contributor.authorWebb, Richard I
dc.contributor.authorSchembri, Mark A
dc.date.accessioned2017-05-03T15:29:30Z
dc.date.available2017-05-03T15:29:30Z
dc.date.issued2007
dc.date.modified2010-02-12T06:42:23Z
dc.identifier.issn1350-0872
dc.identifier.doi10.1099/mic.0.2006/004648-0
dc.identifier.urihttp://hdl.handle.net/10072/27689
dc.description.abstractUropathogenic Escherichia coli (UPEC) is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with virulence of UPEC are fimbrial adhesins, which mediate attachment to specific receptors, enhance persistence and trigger innate host responses. UPEC produce a range of fimbrial adhesins, with type 1 and P fimbriae of the chaperone-usher subclass being the best characterized. The prototype UPEC strain CFT073 contains ten gene clusters that contain genes characteristic of this class of fimbriae. However, only five of these gene clusters have been characterized in detail. In this study the F9 fimbrial gene cluster (c1931-c1936) from CFT073 has been characterized. The F9 fimbriae-encoding genes were PCR amplified, cloned and expressed in a K-12 background devoid of type 1 fimbriae. While F9 fimbrial expression was not associated with any haemagglutination or cellular adherence properties, a role in biofilm formation was observed. E. coli K-12 cells expressing F9 fimbriae produced a dense and uniform biofilm in both microtitre plate and continuous-flow biofilm model systems. In wild-type UPEC CFT073, expression of the F9 major subunit-encoding gene was detected during exponential growth in M9 minimal medium. F9 expression could also be detected following selection and enrichment for pellicle growth in a CFT073fim foc double mutant. The F9 genes appear to be common in UPEC and other types of pathogenic E. coli. However, their precise contribution to disease remains to be determined.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent644006 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherSociety for General Microbiology
dc.publisher.placeUK
dc.publisher.urihttp://mic.sgmjournals.org/
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom2321
dc.relation.ispartofpageto2331
dc.relation.ispartofissue7
dc.relation.ispartofjournalMicrobiology
dc.relation.ispartofvolume153
dc.rights.retentionY
dc.subject.fieldofresearchMedical bacteriology
dc.subject.fieldofresearchcode320701
dc.titleThe Role of F9 Fimbriae of Uropathogenic Escherichia coli in Biofilm Formation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2007 Society for General Microbiology (SGM). This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
gro.date.issued2007
gro.hasfulltextFull Text
gro.griffith.authorUlett, Glen C.


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