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  • Recovery of adhesion to chondroitin-4-sulphate in Plasmodium falciparum varCSA disruption mutants by antigenically similar PfEMP1 variants

    Author(s)
    Andrews, KT
    Pirrit, LA
    Przyborski, JM
    Sanchez, CP
    Sterkers, Y
    Ricken, S
    Wickert, H
    Lepolard, C
    Avril, M
    Scherf, A
    Gysin, R
    Lanzer, M
    Griffith University Author(s)
    Andrews, Katherine T.
    Year published
    2003
    Metadata
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    Abstract
    Protection against maternal malaria has been associated with the acquisition of a specific antibody response that prevents adhesion of Plasmodium falciparum-infected erythrocytes to the glycosaminoglycan chondroitin-4-sulphate (CSA), which is present in the placental intervillous space. These antibodies are directed against variant forms of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) that mediate binding to CSA. We have generated insertional disruption mutants of the gene encoding the CSA-binding phenotype in the P. falciparum clone FCR3 (var CSA) to test the hypothesis that strategies targeting the parasite's ...
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    Protection against maternal malaria has been associated with the acquisition of a specific antibody response that prevents adhesion of Plasmodium falciparum-infected erythrocytes to the glycosaminoglycan chondroitin-4-sulphate (CSA), which is present in the placental intervillous space. These antibodies are directed against variant forms of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) that mediate binding to CSA. We have generated insertional disruption mutants of the gene encoding the CSA-binding phenotype in the P. falciparum clone FCR3 (var CSA) to test the hypothesis that strategies targeting the parasite's determinant for this adhesive phenotype may prevent sequestration of infected erythrocytes in the placenta and hence the development of maternal malaria. The var CSA-disruption mutants were initially unable to adhere to CSA; however, they could recover the phenotype after repeated selection over CSA. We show that recovery of CSA binding is var CSA independent and mediated by the activation of a novel var variant. Importantly, the corresponding PfEMP1 protein reacts with a monoclonal antibody recognizing the DBL3? domain of the var CSA gene product, indicating that the DBL3? CSA-binding domains are conserved between these PfEMP1-binding variants. Our data support strategies exploring these conserved epitopes as vaccine candidates against maternal malaria.
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    Journal Title
    Molecular Microbiology
    Volume
    49
    Issue
    3
    Publisher URI
    http://www.wiley.com/bw/journal.asp?ref=0950-382X
    DOI
    https://doi.org/10.1046/j.1365-2958.2003.03595.x
    Subject
    Biological sciences
    Agricultural, veterinary and food sciences
    Biomedical and clinical sciences
    Publication URI
    http://hdl.handle.net/10072/27824
    Collection
    • Journal articles

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