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dc.contributor.authorHerrera, S
dc.contributor.authorBonelo, A
dc.contributor.authorPerlaza, BL
dc.contributor.authorValencia, AZ
dc.contributor.authorCifuentes, C
dc.contributor.authorHurtado, S
dc.contributor.authorQuintero, G
dc.contributor.authorLopez, JA
dc.contributor.authorCorradin, G
dc.contributor.authorArevalo-Herrera, M
dc.date.accessioned2017-05-03T16:58:40Z
dc.date.available2017-05-03T16:58:40Z
dc.date.issued2004
dc.date.modified2009-12-21T06:45:43Z
dc.identifier.issn0020-7519
dc.identifier.doi10.1016/j.ijpara.2004.10.009
dc.identifier.urihttp://hdl.handle.net/10072/27868
dc.description.abstractThree long synthetic peptides corresponding to amino (N), repeat (R) and carboxyl (C) regions of the Plasmodium vivax circumsporozoite (CS) protein were synthesised and used to assess their potential as vaccine candidates. Antigenicity studies were carried out using human blood samples from residents of a malaria-endemic area of Colombia, and immunogenicity was tested in Aotus monkeys. The N and C peptides spanned the total native amino and carboxyl flanking regions, whereas the R peptide corresponded to a construct based on the first central nona-peptide repeated in tandem three times and colinearly linked to a universal T-cell epitope (ptt-30) derived from tetanus toxin. All three peptides had been shown previously to contain several B-, T-helper (Th) and Cytotoxic T Lymphocytes (CTL) epitopes. Sixty-one percent of the human sera reacted with the R region, whereas 35 and 39% of the samples had antibodies against the N and C peptides, respectively. Human Peripheral Blood Mononuclear Cells (PBMC) showed higher levels of IFN-? than IL-4 when stimulated with peptides containing Th epitopes. Aotus monkeys immunised with the peptides formulated in either Montanide ISA720 or Freund's adjuvants produced strong antibody responses that recognised the peptide immunogens and the native circumsporozoite protein on sporozoites. Additionally, high IFN-? production was induced when Aotus lymphocytes were stimulated in vitro with each of the three peptides. We observed boosting of antibody responses and IFN-? production by exposure to live sporozoites. These results confirm the high antigenicity and immunogenicity of such synthetic polypeptides and underline their vaccine potential.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeUnited States of America
dc.relation.ispartofpagefrom1535
dc.relation.ispartofpageto1546
dc.relation.ispartofissue13-14
dc.relation.ispartofjournalInternational Journal for Parasitology
dc.relation.ispartofvolume34
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchZoology
dc.subject.fieldofresearchVeterinary sciences
dc.subject.fieldofresearchImmunology not elsewhere classified
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode3109
dc.subject.fieldofresearchcode3009
dc.subject.fieldofresearchcode320499
dc.titleUse of long synthetic peptides to study the antigenicity and immunogenicity of the Plasmodium vivax circumsporozoite protein
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2004
gro.hasfulltextNo Full Text
gro.griffith.authorLopez Ramirez, Alejandro


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